Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Phylogenetic-affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic Streptomyces sp. inhabiting an Indian saltpan
Year:
2011
Source of publication :
Journal of Basic Microbiology
Authors :
Satheeja Santhi, Velayudhan
;
.
Volume :
51
Co-Authors:
Arul Jose, P., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Satheeja Santhi, V., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Jebakumar, S.R.D., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Facilitators :
From page:
348
To page:
(
Total pages:
-347
)
Abstract:
A Gram-positive, moderately halophilic Streptomyces strain, designated JAJ06, was isolated from saltpan soil collected at Tuticorin, India, and subjected to a polyphasic characterization with an insight into their biotechnological importance. Growth characteristics and antimicrobial com-pound producing capabilities of Streptomyces sp. JAJ06 were observed on various International Streptomyces Project (ISP) media and production media. Optimum growth was observed on modified ISP 4 medium supplemented with 4% NaCl (w/v) at 29 °C incubated for 7 days. Maximum antibacterial compound production with good mycelial growth was observed on starch-yeast extract-peptone medium prepared with seawater (90%, v/v). The 16S rRNA gene based phylogenetic affiliation was determined by using various bioinformatics tools and the strain was identified as Streptomyces sp. JAJ06 with 99% sequence similarity to Streptomyces radiopugnansT. An antimicrobial assay of antimicrobial compound derived from Streptomyces sp. JAJ06 against a set of bacteria and a yeast strain revealed antimicrobial activity with significant minimal inhibitory concentrations. The potential antimicrobial compound produced by Streptomyces sp. JAJ06 was found to be polyketide in nature. Cloning and sequence analysis of 613-bp fragment of ketosynthase gene from the type-II polyketide operon revealed that Streptomyces sp. JAJ06 has the KSα gene with 91% sequence similarity to the type II polyketide synthase gene of Streptomyces peucetius. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Note:
Related Files :
biosynthesis
Genetics
India
metabolism
Microbiology
molecular genetics
Show More
Related Content
More details
DOI :
10.1002/jobm.201000253
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29696
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:48
You may also be interested in
Scientific Publication
Phylogenetic-affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic Streptomyces sp. inhabiting an Indian saltpan
51
Arul Jose, P., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Satheeja Santhi, V., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Jebakumar, S.R.D., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Phylogenetic-affiliation, antimicrobial potential and PKS gene sequence analysis of moderately halophilic Streptomyces sp. inhabiting an Indian saltpan
A Gram-positive, moderately halophilic Streptomyces strain, designated JAJ06, was isolated from saltpan soil collected at Tuticorin, India, and subjected to a polyphasic characterization with an insight into their biotechnological importance. Growth characteristics and antimicrobial com-pound producing capabilities of Streptomyces sp. JAJ06 were observed on various International Streptomyces Project (ISP) media and production media. Optimum growth was observed on modified ISP 4 medium supplemented with 4% NaCl (w/v) at 29 °C incubated for 7 days. Maximum antibacterial compound production with good mycelial growth was observed on starch-yeast extract-peptone medium prepared with seawater (90%, v/v). The 16S rRNA gene based phylogenetic affiliation was determined by using various bioinformatics tools and the strain was identified as Streptomyces sp. JAJ06 with 99% sequence similarity to Streptomyces radiopugnansT. An antimicrobial assay of antimicrobial compound derived from Streptomyces sp. JAJ06 against a set of bacteria and a yeast strain revealed antimicrobial activity with significant minimal inhibitory concentrations. The potential antimicrobial compound produced by Streptomyces sp. JAJ06 was found to be polyketide in nature. Cloning and sequence analysis of 613-bp fragment of ketosynthase gene from the type-II polyketide operon revealed that Streptomyces sp. JAJ06 has the KSα gene with 91% sequence similarity to the type II polyketide synthase gene of Streptomyces peucetius. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Scientific Publication
נגישות
menu      
You may also be interested in