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Improved electrophoretic resolution of RNA transcripts by specific homologous oligonucleotide gel retardation
Year:
2001
Source of publication :
Journal of Virological Methods
Authors :
Maslenin, Ludmila
;
.
Rosner, Arie
;
.
Volume :
92
Co-Authors:
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1
To page:
4
(
Total pages:
4
)
Abstract:
An improved procedure for the resolution of RNA transcripts by electrophoretic gel retardation, mediated by annealing to specific homologous oligonucleotides is described. The N and NTN strains of PVY served as a model system. Non-polymorphic but sequence-diverse RNA transcripts were copied from PCR products of the two virus strains. The transcripts were resolved by gel electrophoresis, because of the differential retardation effect caused by the binding of strain-specific homologous oligonucleotides. The two PVY strains were thus differentiated. Applicability of this method to virus strain differentiation in general is discussed. © 2001 Elsevier Science B.V.
Note:
Related Files :
Electrophoresis
reverse transcription polymerase chain reaction
Strain differentiation
virus RNA
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More details
DOI :
10.1016/S0166-0934(00)00218-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29712
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
Improved electrophoretic resolution of RNA transcripts by specific homologous oligonucleotide gel retardation
92
Rosner, A., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, ARO, Volcani Center, Bet Dagan 50250, Israel
Improved electrophoretic resolution of RNA transcripts by specific homologous oligonucleotide gel retardation
An improved procedure for the resolution of RNA transcripts by electrophoretic gel retardation, mediated by annealing to specific homologous oligonucleotides is described. The N and NTN strains of PVY served as a model system. Non-polymorphic but sequence-diverse RNA transcripts were copied from PCR products of the two virus strains. The transcripts were resolved by gel electrophoresis, because of the differential retardation effect caused by the binding of strain-specific homologous oligonucleotides. The two PVY strains were thus differentiated. Applicability of this method to virus strain differentiation in general is discussed. © 2001 Elsevier Science B.V.
Scientific Publication
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