נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Direct evidence for the lack of methylation of two pulse labeled plant RNAs
Year:
1974
Source of publication :
Plant and Cell Physiology
Authors :
Rosner, Arie
;
.
Volume :
15
Co-Authors:
Posner, H.B., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Rosner, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
807
To page:
811
(
Total pages:
5
)
Abstract:
A product of the processing of precursor rRNA ("excess" RNA) has been indirectly found to be unmethylated in mammalian systems, but direct measurement was precluded because of its instability. The "excess" RNA of duckweeds is relatively stable allowing direct estimation of its methylation by polyacrylamide gel electrophoresis. The "excess" RNA with an apparent molecular weight 0.5×106 was unmethylated. A pulse labeled RNA with an apparent molecular weight of 1.2×106 (presumably from chloroplasts) was also unmethylated. Under similar conditions the presumed cytoplasmic rRNA precursors, and the mature cytoplasmic and chloroplast rRNAs were methylated. © 1974 Oxford University Press.
Note:
Related Files :
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29720
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
Scientific Publication
Direct evidence for the lack of methylation of two pulse labeled plant RNAs
15
Posner, H.B., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Rosner, A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Direct evidence for the lack of methylation of two pulse labeled plant RNAs
A product of the processing of precursor rRNA ("excess" RNA) has been indirectly found to be unmethylated in mammalian systems, but direct measurement was precluded because of its instability. The "excess" RNA of duckweeds is relatively stable allowing direct estimation of its methylation by polyacrylamide gel electrophoresis. The "excess" RNA with an apparent molecular weight 0.5×106 was unmethylated. A pulse labeled RNA with an apparent molecular weight of 1.2×106 (presumably from chloroplasts) was also unmethylated. Under similar conditions the presumed cytoplasmic rRNA precursors, and the mature cytoplasmic and chloroplast rRNAs were methylated. © 1974 Oxford University Press.
Scientific Publication
You may also be interested in