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Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens (Journal of Experimental Biology)
Year:
2014
Source of publication :
Journal of Experimental Biology
Authors :
Friedman-Einat, Miriam
;
.
Ruzal, Mark
;
.
Seroussi, Eyal
;
.
Shinder, Dmitry A.
;
.
Yosefi, Sara
;
.
Volume :
217
Co-Authors:

Gertler, A., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shpilman, M., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Rosenblum, C.I., Merck and Company Inc., Whitehouse Station (C.I.R.), NJ 07065, United States
 

Facilitators :
From page:
180
To page:
184
(
Total pages:
5
)
Abstract:
A chicken gene orthologous to human leptin receptor (LEPR) has been characterized and found to be active in leptin signaling in vitro in response to a variety of recombinant leptins and leptin-containing blood samples. However, the endogenous ligand of chicken LEPR (cLEPR) - the putative chicken leptin - has been reported by us and others to be undetectable at the DNA, mRNA, protein and activity levels. These reports have raised questions as to cLEPR's role. Here we analyzed the effects of a pegylated superactive mouse leptin antagonist (PEG-SMLA) in chicken. We showed that the leptin antagonist efficiently and specifically blocks leptin signaling through the cLEPR in vitro. The effect of the leptin antagonist was then studied in vivo by daily administration of 10 mg kg-1 for 10 consecutive days to white leghorn female chickens (Gallus gallus) at the age of 2 weeks. Despites the efficient attenuation of the cLEPR in vitro, no effect was observed on body mass, feed intake, feed efficiency or fat accumulation in the treated birds. Because similar treatment in rodents leads to a highly pronounced increase in appetite and body mass that are observed from the first day of treatment, it is concluded that the cLEPR is not implicated in the control of appetite or adipose homeostasis in chickens. © 2014. Published by The Company of Biologists Ltd.
Note:
Related Files :
Chicken leptin
Chicken leptin receptor
energy balance
Leptin antagonist
Show More
Related Content
More details
DOI :
7
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29723
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
Scientific Publication
Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens (Journal of Experimental Biology)
217

Gertler, A., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Shpilman, M., Faculty of Agriculture, Food and Environment, Hebrew University, Rehovot 76100, Israel
Rosenblum, C.I., Merck and Company Inc., Whitehouse Station (C.I.R.), NJ 07065, United States
 

Pegylated leptin antagonist with strong orexigenic activity in mice is not effective in chickens
A chicken gene orthologous to human leptin receptor (LEPR) has been characterized and found to be active in leptin signaling in vitro in response to a variety of recombinant leptins and leptin-containing blood samples. However, the endogenous ligand of chicken LEPR (cLEPR) - the putative chicken leptin - has been reported by us and others to be undetectable at the DNA, mRNA, protein and activity levels. These reports have raised questions as to cLEPR's role. Here we analyzed the effects of a pegylated superactive mouse leptin antagonist (PEG-SMLA) in chicken. We showed that the leptin antagonist efficiently and specifically blocks leptin signaling through the cLEPR in vitro. The effect of the leptin antagonist was then studied in vivo by daily administration of 10 mg kg-1 for 10 consecutive days to white leghorn female chickens (Gallus gallus) at the age of 2 weeks. Despites the efficient attenuation of the cLEPR in vitro, no effect was observed on body mass, feed intake, feed efficiency or fat accumulation in the treated birds. Because similar treatment in rodents leads to a highly pronounced increase in appetite and body mass that are observed from the first day of treatment, it is concluded that the cLEPR is not implicated in the control of appetite or adipose homeostasis in chickens. © 2014. Published by The Company of Biologists Ltd.
Scientific Publication
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