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Calcium antagonists inhibit bending and differential ethylene production of gravistimulated Ornithogalum 'Nova' cut flower spikes
Year:
2005
Source of publication :
Postharvest Biology and Technology
Authors :
Friedman, Haya
;
.
Meir, Shimon
;
.
Philosoph-Hadas, Sonia
;
.
Rosenberger, Ida
;
.
Volume :
36
Co-Authors:
Friedman, H., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Meir, S., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rosenberger, I., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Halevy, A.H., Robert H. Smith Inst. Plant Sci. G., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Philosoph-Hadas, S., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
9
To page:
20
(
Total pages:
12
)
Abstract:
To study the role of calcium ions in the gravitropic response of cut flowering shoots, we have examined the effects of various calcium modulators on stem bending of Ornithogalum spp. 'Nova' spikes following dry or wet gravistimulation. The most effective calcium chelators were further examined for their effects on the gravity- or IAA-induced ethylene formation, which represent signal transduction events in the gravity signalling pathway. The calcium chelators 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′- tetraacetic acid (BAPTA), ethylene glycol bis(β-aminoethylether) N,N,N′,N′-tetraacetic acid (EGTA), trans-1,2-cyclohexane dinitro-N,N,N′,N′-tetraacetic acid (CDTA), ethylenediamine- tetraacetic acid (EDTA), and the calcium channel blocker LaCl3, inhibited the gravitropic bending of Ornithogalum 'Nova' spikes. This bending inhibition was obtained following stem gravistimulation both under dry transport (48 h dark at 6°C) or laboratory (24 h light at 20°C) conditions. On the other hand, the calcium ionophore A23187 enhanced the gravitropic response, further supporting the role of intracellular calcium in the gravitropic bending response. These calcium modulators had no deleterious effects on the flowering shoot, indicating that their inhibitory effect is not due to damage. An ethylene gradient was developed across the gravistimulated flowering shoot prior to the visual bending response, in favour of the lower stem flank. This was correlated with higher levels of 1-aminocylclopropane-1-carboxylic acid (ACC) and 1-(malonylamino)-cyclopropane-1-carboxylic acid (MACC) at the lower flank, indicating that the gravitropic stimulus may either lead to activation of ethylene biosynthesis enzymes or to lateral ACC movement. The ethylene gradient was abolished following spikes pulsing with EGTA or CDTA, which prevented the increased ethylene production at the lower flank. CDTA also abolished the IAA-induced ethylene production in vertical stems. This may imply that the calcium chelators inhibit ethylene production of the lower flank via inhibition of IAA action, which induces increased ethylene formation. This indicates that the calcium chelators act upstream to activation of ethylene biosynthesis, probably by abolishing earlier gravity-related processes. Our results further support our previous findings in snapdragon that calcium may act as an important mediator of the gravitropic signal transduction mechanism in cut flowering shoots. © 2004 Elsevier B.V. All rights reserved.
Note:
Related Files :
Antirrhinum majus
Calcium chelators
ethylene
Gravitropism
LaCl3
Ornithogalum
Ornithogalum 'Nova'
Stem bending
Show More
Related Content
More details
DOI :
10.1016/j.postharvbio.2004.11.002
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29768
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
Calcium antagonists inhibit bending and differential ethylene production of gravistimulated Ornithogalum 'Nova' cut flower spikes
36
Friedman, H., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Meir, S., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rosenberger, I., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Halevy, A.H., Robert H. Smith Inst. Plant Sci. G., Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot 76100, Israel
Philosoph-Hadas, S., Dept. Postharvest Sci. Fresh Produce, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Calcium antagonists inhibit bending and differential ethylene production of gravistimulated Ornithogalum 'Nova' cut flower spikes
To study the role of calcium ions in the gravitropic response of cut flowering shoots, we have examined the effects of various calcium modulators on stem bending of Ornithogalum spp. 'Nova' spikes following dry or wet gravistimulation. The most effective calcium chelators were further examined for their effects on the gravity- or IAA-induced ethylene formation, which represent signal transduction events in the gravity signalling pathway. The calcium chelators 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′- tetraacetic acid (BAPTA), ethylene glycol bis(β-aminoethylether) N,N,N′,N′-tetraacetic acid (EGTA), trans-1,2-cyclohexane dinitro-N,N,N′,N′-tetraacetic acid (CDTA), ethylenediamine- tetraacetic acid (EDTA), and the calcium channel blocker LaCl3, inhibited the gravitropic bending of Ornithogalum 'Nova' spikes. This bending inhibition was obtained following stem gravistimulation both under dry transport (48 h dark at 6°C) or laboratory (24 h light at 20°C) conditions. On the other hand, the calcium ionophore A23187 enhanced the gravitropic response, further supporting the role of intracellular calcium in the gravitropic bending response. These calcium modulators had no deleterious effects on the flowering shoot, indicating that their inhibitory effect is not due to damage. An ethylene gradient was developed across the gravistimulated flowering shoot prior to the visual bending response, in favour of the lower stem flank. This was correlated with higher levels of 1-aminocylclopropane-1-carboxylic acid (ACC) and 1-(malonylamino)-cyclopropane-1-carboxylic acid (MACC) at the lower flank, indicating that the gravitropic stimulus may either lead to activation of ethylene biosynthesis enzymes or to lateral ACC movement. The ethylene gradient was abolished following spikes pulsing with EGTA or CDTA, which prevented the increased ethylene production at the lower flank. CDTA also abolished the IAA-induced ethylene production in vertical stems. This may imply that the calcium chelators inhibit ethylene production of the lower flank via inhibition of IAA action, which induces increased ethylene formation. This indicates that the calcium chelators act upstream to activation of ethylene biosynthesis, probably by abolishing earlier gravity-related processes. Our results further support our previous findings in snapdragon that calcium may act as an important mediator of the gravitropic signal transduction mechanism in cut flowering shoots. © 2004 Elsevier B.V. All rights reserved.
Scientific Publication
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