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The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene
Year:
2003
Source of publication :
Theoretical and Applied Genetics
Authors :
Frankel, Pnina
;
.
Gilboa, Nehama
;
.
Lalazar, Avraham
;
.
Levin, Ilan
;
.
Tanny, Sophie
;
.
Volume :
106
Co-Authors:
Levin, I., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Frankel, P., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tanny, S., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lalazar, A., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
454
To page:
460
(
Total pages:
7
)
Abstract:
A comprehensive, multi-generation, allele test, carried out in this study, suggests that the tomato mutations dark-green (dg) and high pigment 2j (hp-2j) are allelic. The hp-2j mutant is caused by a mutation in the tomato homolog of the DEETIOLATED1 (DET1) gene, involved in the signal transduction cascade of light perception and morphogenesis. This suggestion is in agreement with the exaggerated photomorphogenic deetiolation response of homozygous dg mutants grown under modulated light conditions. Sequence analysis of the DET1 gene was carried out in dg mutants representing two different lines, and revealed a single A-to-T base transversion in the second exon of the DET1 gene in comparison with the normal wild-type sequence. This transversion results in a conserved Asparagine34-to-Isoleucine34 amino-acid substitution, and eliminates a recognition site for the AclI restriction endonuclease, present in the wild-type and in the other currently known tomato mutants at the DET1 locus. This polymorphism was used to develop a PCR-based DNA marker, which enables an early genotypic selection for breeding lycopene-rich tomatoes. Using this marker and sequence analysis we demonstrate that an identical base transversion also exists in dg mutants of the cultivar Manapal, in which the natural dg mutation was originally discovered. A linkage analysis, carried out in a F2 population, shows a very strong linkage association between the DET1 locus of dg mutant plants and the photomorphogenic response of the seedlings, measured as hypocotyl length (12 < LOD Score < 13, R2 = 51.1%). The results presented in this study strongly support the hypothesis that the tomato dg mutation is a novel allele of the tomato homolog of the DET1 gene.
Note:
Related Files :
Base Sequence
Crops
genetic engineering
genetic markers
light
lycopene
Molecular Sequence Data
mutation
phenotype
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29777
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene
106
Levin, I., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Frankel, P., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gilboa, N., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tanny, S., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Lalazar, A., Dept. of Plant Genetics and Breeding, Inst. of Plant Fld. and Garden Crops, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene
A comprehensive, multi-generation, allele test, carried out in this study, suggests that the tomato mutations dark-green (dg) and high pigment 2j (hp-2j) are allelic. The hp-2j mutant is caused by a mutation in the tomato homolog of the DEETIOLATED1 (DET1) gene, involved in the signal transduction cascade of light perception and morphogenesis. This suggestion is in agreement with the exaggerated photomorphogenic deetiolation response of homozygous dg mutants grown under modulated light conditions. Sequence analysis of the DET1 gene was carried out in dg mutants representing two different lines, and revealed a single A-to-T base transversion in the second exon of the DET1 gene in comparison with the normal wild-type sequence. This transversion results in a conserved Asparagine34-to-Isoleucine34 amino-acid substitution, and eliminates a recognition site for the AclI restriction endonuclease, present in the wild-type and in the other currently known tomato mutants at the DET1 locus. This polymorphism was used to develop a PCR-based DNA marker, which enables an early genotypic selection for breeding lycopene-rich tomatoes. Using this marker and sequence analysis we demonstrate that an identical base transversion also exists in dg mutants of the cultivar Manapal, in which the natural dg mutation was originally discovered. A linkage analysis, carried out in a F2 population, shows a very strong linkage association between the DET1 locus of dg mutant plants and the photomorphogenic response of the seedlings, measured as hypocotyl length (12 < LOD Score < 13, R2 = 51.1%). The results presented in this study strongly support the hypothesis that the tomato dg mutation is a novel allele of the tomato homolog of the DET1 gene.
Scientific Publication
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