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Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations
Year:
2013
Source of publication :
BMC Genomics
Authors :
Quansah, Lydia
;
.
Volume :
14
Co-Authors:
Crosatti, C., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Quansah, L., Jacob Blaustein Institutes for Desert Research, French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990 Sde Boqer, Israel
Maré, C., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Giusti, L., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Roncaglia, E., Center for Genome Research, Biomedical Sciences Department, Biological Chemistry Section, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
Atienza, S.G., Departamento de Mejora Genética, IAS-CSIC, Apdo. 4084, Cordoba 14980, Spain
Cattivelli, L., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Fait, A., Jacob Blaustein Institutes for Desert Research, French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990 Sde Boqer, Israel
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background: Alloplasmic lines provide a unique tool to study nuclear-cytoplasmic interactions. Three alloplasmic lines, with nuclear genomes from Triticum aestivum and harboring cytoplasm from Aegilops uniaristata, Aegilops tauschii and Hordeum chilense, were investigated by transcript and metabolite profiling to identify the effects of cytoplasmic substitution on nuclear-cytoplasmic signaling mechanisms.Results: In combining the wheat nuclear genome with a cytoplasm of H. chilense, 540 genes were significantly altered, whereas 11 and 28 genes were significantly changed in the alloplasmic lines carrying the cytoplasm of Ae. uniaristata or Ae. tauschii, respectively. We identified the RNA maturation-related process as one of the most sensitive to a perturbation of the nuclear-cytoplasmic interaction. Several key components of the ROS chloroplast retrograde signaling, together with the up-regulation of the ROS scavenging system, showed that changes in the chloroplast genome have a direct impact on nuclear-cytoplasmic cross-talk. Remarkably, the H. chilense alloplasmic line down-regulated some genes involved in the determination of cytoplasmic male sterility without expressing the male sterility phenotype. Metabolic profiling showed a comparable response of the central metabolism of the alloplasmic and euplasmic lines to light, while exposing larger metabolite alterations in the H. chilense alloplasmic line as compared with the Aegilops lines, in agreement with the transcriptomic data. Several stress-related metabolites, remarkably raffinose, were altered in content in the H. chilense alloplasmic line when exposed to high light, while amino acids, as well as organic acids were significantly decreased. Alterations in the levels of transcript, related to raffinose, and the photorespiration-related metabolisms were associated with changes in the level of related metabolites.Conclusion: The replacement of a wheat cytoplasm with the cytoplasm of a related species affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations. The extent of these modifications was limited in the alloplasmic lines with Aegilops cytoplasm, and more evident in the alloplasmic line with H. chilense cytoplasm. We consider that, this finding might be linked to the phylogenetic distance of the genomes. © 2013 Crosatti et al.; licensee BioMed Central Ltd.
Note:
Related Files :
fatty acids
Hordeum chilense
metabolome
Metabolomics
photosynthesis
Plant Genome
Triticum
Triticum aestivum
Show More
Related Content
More details
DOI :
10.1186/1471-2164-14-868
Article number:
868
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29842
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:49
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Scientific Publication
Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations
14
Crosatti, C., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Quansah, L., Jacob Blaustein Institutes for Desert Research, French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990 Sde Boqer, Israel
Maré, C., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Giusti, L., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Roncaglia, E., Center for Genome Research, Biomedical Sciences Department, Biological Chemistry Section, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
Atienza, S.G., Departamento de Mejora Genética, IAS-CSIC, Apdo. 4084, Cordoba 14980, Spain
Cattivelli, L., Consiglio per la Ricerca e la Sperimentazione in Agricoltura -Genomics Research Centre, via S. Protaso 302, 29017 Fiorenzuola d' Arda, (PC), Italy
Fait, A., Jacob Blaustein Institutes for Desert Research, French Associates Institute for Agriculture and Biotechnology of Drylands, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, 84990 Sde Boqer, Israel
Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations
Background: Alloplasmic lines provide a unique tool to study nuclear-cytoplasmic interactions. Three alloplasmic lines, with nuclear genomes from Triticum aestivum and harboring cytoplasm from Aegilops uniaristata, Aegilops tauschii and Hordeum chilense, were investigated by transcript and metabolite profiling to identify the effects of cytoplasmic substitution on nuclear-cytoplasmic signaling mechanisms.Results: In combining the wheat nuclear genome with a cytoplasm of H. chilense, 540 genes were significantly altered, whereas 11 and 28 genes were significantly changed in the alloplasmic lines carrying the cytoplasm of Ae. uniaristata or Ae. tauschii, respectively. We identified the RNA maturation-related process as one of the most sensitive to a perturbation of the nuclear-cytoplasmic interaction. Several key components of the ROS chloroplast retrograde signaling, together with the up-regulation of the ROS scavenging system, showed that changes in the chloroplast genome have a direct impact on nuclear-cytoplasmic cross-talk. Remarkably, the H. chilense alloplasmic line down-regulated some genes involved in the determination of cytoplasmic male sterility without expressing the male sterility phenotype. Metabolic profiling showed a comparable response of the central metabolism of the alloplasmic and euplasmic lines to light, while exposing larger metabolite alterations in the H. chilense alloplasmic line as compared with the Aegilops lines, in agreement with the transcriptomic data. Several stress-related metabolites, remarkably raffinose, were altered in content in the H. chilense alloplasmic line when exposed to high light, while amino acids, as well as organic acids were significantly decreased. Alterations in the levels of transcript, related to raffinose, and the photorespiration-related metabolisms were associated with changes in the level of related metabolites.Conclusion: The replacement of a wheat cytoplasm with the cytoplasm of a related species affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations. The extent of these modifications was limited in the alloplasmic lines with Aegilops cytoplasm, and more evident in the alloplasmic line with H. chilense cytoplasm. We consider that, this finding might be linked to the phylogenetic distance of the genomes. © 2013 Crosatti et al.; licensee BioMed Central Ltd.
Scientific Publication
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