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mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
Year:
2007
Source of publication :
Molecular and Cellular Biology
Authors :
Zipor, Gadi
;
.
Volume :
27
Co-Authors:
Aronov, S., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gelin-Licht, R., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Haim, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Safran, E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
3441
To page:
3455
(
Total pages:
15
)
Abstract:
Polarized growth in the budding yeast Saccharomyces cerevisiae depends upon the asymmetric localization and enrichment of polarity and secretion factors at the membrane prior to budding. We examined how these factors (i.e., Cdc42, Sec4, and Sro7) reach the bud site and found that their respective mRNAs localize to the tip of the incipient bud prior to nuclear division. Asymmetric mRNA localization depends upon factors that facilitate ASH1 mRNA localization (e.g., the 3′ untranslated region, She proteins 1 to 5, Puf6, actin cytoskeleton, and a physical association with She2). mRNA placement precedes protein enrichment and subsequent bud emergence, implying that mRNA localization contributes to polarization. Correspondingly, mRNAs encoding proteins which are not asymmetrically distributed (i.e., Snc1, Mso1, Tub1, Pex3, and Oxa1) are not polarized. Finally, mutations which affect cortical endoplasmic reticulum (ER) entry and anchoring in the bud (myo4Δ, sec3Δ, and srp101) also affect asymmetric mRNA localization. Bud-localized mRNAs, including ASH1, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Note:
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More details
DOI :
10.1128/MCB.01643-06
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29881
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
Scientific Publication
mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
27
Aronov, S., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gelin-Licht, R., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Zipor, G., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Haim, L., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Safran, E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Gerst, J.E., Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae
Polarized growth in the budding yeast Saccharomyces cerevisiae depends upon the asymmetric localization and enrichment of polarity and secretion factors at the membrane prior to budding. We examined how these factors (i.e., Cdc42, Sec4, and Sro7) reach the bud site and found that their respective mRNAs localize to the tip of the incipient bud prior to nuclear division. Asymmetric mRNA localization depends upon factors that facilitate ASH1 mRNA localization (e.g., the 3′ untranslated region, She proteins 1 to 5, Puf6, actin cytoskeleton, and a physical association with She2). mRNA placement precedes protein enrichment and subsequent bud emergence, implying that mRNA localization contributes to polarization. Correspondingly, mRNAs encoding proteins which are not asymmetrically distributed (i.e., Snc1, Mso1, Tub1, Pex3, and Oxa1) are not polarized. Finally, mutations which affect cortical endoplasmic reticulum (ER) entry and anchoring in the bud (myo4Δ, sec3Δ, and srp101) also affect asymmetric mRNA localization. Bud-localized mRNAs, including ASH1, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Scientific Publication
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