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Transcriptome sequence analysis of an ornamental plant, ananas comosus var. Bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
Year:
2015
Source of publication :
PLoS ONE
Authors :
Kanakala, Surapathrudu
;
.
Volume :
10
Co-Authors:
Ma, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Kanakala, S., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
He, Y., Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China
Zhang, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Zhong, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Background Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus highthroughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies. Results The Ananas comosus var. bracteatus transcriptome was sequenced by the Illumina pairedend sequencing technology. We obtained a total of 23.5 million high quality sequencing reads, 1,555,808 contigs and 41,052 unigenes. In total 41,052 unigenes of Ananas comosus var. bracteatus, 23,275 unigenes were annotated in the NCBI non-redundant protein database and 23,134 unigenes were annotated in the Swiss-Port database. Out of these, 17,748 and 8,505 unigenes were assigned to gene ontology categories and clusters of orthologous groups, respectively. Functional annotation against Kyoto Encyclopedia of Genes and Genomes Pathway database identified 5,825 unigenes which were mapped to 117 pathways. The assembly predicted many unigenes that were previously unknown. The annotated unigenes were compared against pineapple, rice, maize, Arabidopsis, and sorghum. Unigenes that did not match any of those five sequence datasets are considered to be Ananas comosus var. bracteatus unique. We predicted unigenes encoding enzymes involved in terpenoid and phenylpropanoid biosynthesis. Conclusion The sequence data provide the most comprehensive transcriptomic resource currently available for Ananas comosus var. bracteatus. To our knowledge; this is the first report on the de novo transcriptome sequencing of the Ananas comosus var. bracteatus. Unigenes obtained in this study, may help improve future gene expression, genetic and genomics studies in Ananas comosus var. bracteatus. © 2015 Ma et al.
Note:
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More details
DOI :
10.1371/journal.pone.0119153
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29913
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
Scientific Publication
Transcriptome sequence analysis of an ornamental plant, ananas comosus var. Bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
10
Ma, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Kanakala, S., Institute of Plant Protection, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
He, Y., Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China
Zhang, J., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Zhong, X., College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China
Transcriptome sequence analysis of an ornamental plant, ananas comosus var. Bracteatus, revealed the potential unigenes involved in terpenoid and phenylpropanoid biosynthesis
Background Ananas comosus var. bracteatus (Red Pineapple) is an important ornamental plant for its colorful leaves and decorative red fruits. Because of its complex genome, it is difficult to understand the molecular mechanisms involved in the growth and development. Thus highthroughput transcriptome sequencing of Ananas comosus var. bracteatus is necessary to generate large quantities of transcript sequences for the purpose of gene discovery and functional genomic studies. Results The Ananas comosus var. bracteatus transcriptome was sequenced by the Illumina pairedend sequencing technology. We obtained a total of 23.5 million high quality sequencing reads, 1,555,808 contigs and 41,052 unigenes. In total 41,052 unigenes of Ananas comosus var. bracteatus, 23,275 unigenes were annotated in the NCBI non-redundant protein database and 23,134 unigenes were annotated in the Swiss-Port database. Out of these, 17,748 and 8,505 unigenes were assigned to gene ontology categories and clusters of orthologous groups, respectively. Functional annotation against Kyoto Encyclopedia of Genes and Genomes Pathway database identified 5,825 unigenes which were mapped to 117 pathways. The assembly predicted many unigenes that were previously unknown. The annotated unigenes were compared against pineapple, rice, maize, Arabidopsis, and sorghum. Unigenes that did not match any of those five sequence datasets are considered to be Ananas comosus var. bracteatus unique. We predicted unigenes encoding enzymes involved in terpenoid and phenylpropanoid biosynthesis. Conclusion The sequence data provide the most comprehensive transcriptomic resource currently available for Ananas comosus var. bracteatus. To our knowledge; this is the first report on the de novo transcriptome sequencing of the Ananas comosus var. bracteatus. Unigenes obtained in this study, may help improve future gene expression, genetic and genomics studies in Ananas comosus var. bracteatus. © 2015 Ma et al.
Scientific Publication
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