נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Long-distance transport of mRNA via parenchyma cells and phloem across the host-parasite junction in Cuscuta
Year:
2008
Source of publication :
New Phytologist
Authors :
David-Schwartz, Rakefet
;
.
Volume :
179
Co-Authors:
David-Schwartz, R., Section of Plant Biology, University of California, Davis, CA 95616, United States
Runo, S., Section of Plant Biology, University of California, Davis, CA 95616, United States, Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, Nairobi, Kenya
Townsley, B., Section of Plant Biology, University of California, Davis, CA 95616, United States
MacHuka, J., Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, Nairobi, Kenya
Sinha, N., Section of Plant Biology, University of California, Davis, CA 95616, United States
Facilitators :
From page:
1133
To page:
1141
(
Total pages:
9
)
Abstract:
• It has been shown that the parasitic plant dodder (Cuscuta pentagona) establishes a continuous vascular system through which water and nutrients are drawn. Along with solutes, viruses and proteins, mRNA transcripts are transported from the host to the parasite. The path of the transcripts and their stability in the parasite have yet to be revealed. • To discover the route of mRNA transportation, the in situ reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to locally amplify host transcript within parasitic tissue. The stability of host mRNA molecules was also checked by monitoring specific transcripts along the growing dodder thread. • Four mRNAs, α and β subunits of PYROPHOSPHATE (PPi)-DEPENDENT PHOSPHOFRUCTOKINASE (LePFP), the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), and GIBBERELLIC ACID INSENSITIVE (LeGAI), were found to move from host (tomato (Solanum lycopersicum)) to dodder. LePFP mRNA was localized to the dodder parenchyma cells and to the phloem. LePFP transcripts were found in the growing dodder stem up to 30 cm from the tomato-dodder connection. • These results suggest that mRNA molecules are transferred from host to parasite via symplastic connections between parenchyma cells, move towards the phloem, and are stable for a long distance in the parasite. This may allow developmental coordination between the parasite and its host. © The Authors (2008).
Note:
Related Files :
Cuscuta
Genetics
Medicago sativa
metabolism
Parasite
Parasitology
RNA
Solanum
ultrastructure
Show More
Related Content
More details
DOI :
10.1111/j.1469-8137.2008.02540.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29919
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
Scientific Publication
Long-distance transport of mRNA via parenchyma cells and phloem across the host-parasite junction in Cuscuta
179
David-Schwartz, R., Section of Plant Biology, University of California, Davis, CA 95616, United States
Runo, S., Section of Plant Biology, University of California, Davis, CA 95616, United States, Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, Nairobi, Kenya
Townsley, B., Section of Plant Biology, University of California, Davis, CA 95616, United States
MacHuka, J., Department of Biochemistry and Biotechnology, Kenyatta University, P.O. Box 43844, Nairobi, Kenya
Sinha, N., Section of Plant Biology, University of California, Davis, CA 95616, United States
Long-distance transport of mRNA via parenchyma cells and phloem across the host-parasite junction in Cuscuta
• It has been shown that the parasitic plant dodder (Cuscuta pentagona) establishes a continuous vascular system through which water and nutrients are drawn. Along with solutes, viruses and proteins, mRNA transcripts are transported from the host to the parasite. The path of the transcripts and their stability in the parasite have yet to be revealed. • To discover the route of mRNA transportation, the in situ reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to locally amplify host transcript within parasitic tissue. The stability of host mRNA molecules was also checked by monitoring specific transcripts along the growing dodder thread. • Four mRNAs, α and β subunits of PYROPHOSPHATE (PPi)-DEPENDENT PHOSPHOFRUCTOKINASE (LePFP), the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), and GIBBERELLIC ACID INSENSITIVE (LeGAI), were found to move from host (tomato (Solanum lycopersicum)) to dodder. LePFP mRNA was localized to the dodder parenchyma cells and to the phloem. LePFP transcripts were found in the growing dodder stem up to 30 cm from the tomato-dodder connection. • These results suggest that mRNA molecules are transferred from host to parasite via symplastic connections between parenchyma cells, move towards the phloem, and are stable for a long distance in the parasite. This may allow developmental coordination between the parasite and its host. © The Authors (2008).
Scientific Publication
You may also be interested in