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Genetic diversity in wild and laboratory populations of Heterorhabditis bacteriophora as determined by RAPD-PCR analysis
Year:
1997
Source of publication :
Fundamental and Applied Nematology
Authors :
Glazer, Itamar
;
.
Shapiro, David I.
;
.
Volume :
20
Co-Authors:
Shapiro, D.I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel, Integrated BioControl Systems, Inc., Lawrenceburg, IN 47025, United States
Glazer, I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel
Segal, D., Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
581
To page:
585
(
Total pages:
5
)
Abstract:
Genetic variation in laboratory reared biocontrol agents may be reduced due to founder effect, inbreeding, and selection. We used random amplified polymorphic DNA (RAPD-PCR) to compare genetic variation in two strains of Heterorhabditis bacteriophora. One strain (IS5) was recently isolated from the field and the other strain (HP88) has been reared under laboratory conditions for over 10 years. For each strain, fifteen inbred lines were generated by eight cycles of selfing of a single hermaphrodite (reaching > 90 % homozygosity). Genomic DNA from each of the inbred lines was screened with fourteen decamer primers. Genetic variation was calculated based on average percentage similarity of DNA banding patterns and cluster analysis. The level of within population variation detected did not differ significantly between the two strains.
Note:
Related Files :
biological control
Diversity
Entomopathogenic nematodes
genetic variation
Heterorhabditis bacteriophora
Nematoda
RAPD
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:

Itamar's own PDF.

ID:
29951
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:50
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Scientific Publication
Genetic diversity in wild and laboratory populations of Heterorhabditis bacteriophora as determined by RAPD-PCR analysis
20
Shapiro, D.I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel, Integrated BioControl Systems, Inc., Lawrenceburg, IN 47025, United States
Glazer, I., Department of Nematology, Volcani Center, Bet Dagan, 50-250, Israel
Segal, D., Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel
Genetic diversity in wild and laboratory populations of Heterorhabditis bacteriophora as determined by RAPD-PCR analysis
Genetic variation in laboratory reared biocontrol agents may be reduced due to founder effect, inbreeding, and selection. We used random amplified polymorphic DNA (RAPD-PCR) to compare genetic variation in two strains of Heterorhabditis bacteriophora. One strain (IS5) was recently isolated from the field and the other strain (HP88) has been reared under laboratory conditions for over 10 years. For each strain, fifteen inbred lines were generated by eight cycles of selfing of a single hermaphrodite (reaching > 90 % homozygosity). Genomic DNA from each of the inbred lines was screened with fourteen decamer primers. Genetic variation was calculated based on average percentage similarity of DNA banding patterns and cluster analysis. The level of within population variation detected did not differ significantly between the two strains.
Scientific Publication
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