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Recovery of Virus-Free almond plants by improved in vitro Shoot-Tip Grafting
Year:
2001
Source of publication :
Acta Horticulturae
Authors :
Spiegel, Sara
;
.
Volume :
550
Co-Authors:

Rizqi, A., Unité de Contrôle des Plants, Direction des Domaines Agricoles, Dar Es Salam, Rabat 10000, Morocco
Zemzami, M., Unité de Contrôle des Plants, Direction des Domaines Agricoles, Dar Es Salam, Rabat 10000, Morocco
 

Facilitators :
From page:
447
To page:
453
(
Total pages:
7
)
Abstract:
Shoot-tip grafting (STG) involves aseptic, in vitro grafting of excised shoot-tips from infected plants onto decapitated seedling rootstocks followed by growth of the scions to develop plant material free of virus. STG has been used in certification schemes of citrus, however, for Prunus species an optimization of this technique is required. In the present study, several parameters affecting success of STG were studied with almond plants infected with the ilarviruses prunus necrotic ringspot (PNRSV) and prune dwarf (PDV). Green cuttings harvested from greenhouse-grown plants were used successfully as rootstocks for STG instead of in vitro germinated seedlings. Peach GF305 and pecher de Missour (origin Morocco) were superior to other rootstocks. The use of green cuttings shortened and simplified considerably the traditional rootstock preparation procedure. Various sizes of shoot-tips excised from almond varieties were grafted in vitro and regeneration rates were determined. About 50% graft success was obtained with shoottips consisting of a meristematic dome and three to four leaf primordia. 'STG plants' consisting of the in vitro rootstocks and developed shoot tips (almond variety), were side-grafted in the greenhouse onto vigorous GF305 plants. Rapid growth of the grafted STG plants was observed regardless of the season. Virus status was determined in side-grafted STG plants as soon as leaf material could be collected for an ELISA / PCR assay. Scions found free of the tested viruses were kept and following further development and repeated testing could be harvested for propagation. With these results, an improvement of the STG technique was obtained for almond sanitation. The study is ongoing and its application for other Prunus species will be attempted.
Note:
Related Files :
PDV
PNRSV
STG
Virus elimination
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
29971
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
Scientific Publication
Recovery of Virus-Free almond plants by improved in vitro Shoot-Tip Grafting
550

Rizqi, A., Unité de Contrôle des Plants, Direction des Domaines Agricoles, Dar Es Salam, Rabat 10000, Morocco
Zemzami, M., Unité de Contrôle des Plants, Direction des Domaines Agricoles, Dar Es Salam, Rabat 10000, Morocco
 

Recovery of Virus-Free almond plants by improved in vitro Shoot-Tip Grafting
Shoot-tip grafting (STG) involves aseptic, in vitro grafting of excised shoot-tips from infected plants onto decapitated seedling rootstocks followed by growth of the scions to develop plant material free of virus. STG has been used in certification schemes of citrus, however, for Prunus species an optimization of this technique is required. In the present study, several parameters affecting success of STG were studied with almond plants infected with the ilarviruses prunus necrotic ringspot (PNRSV) and prune dwarf (PDV). Green cuttings harvested from greenhouse-grown plants were used successfully as rootstocks for STG instead of in vitro germinated seedlings. Peach GF305 and pecher de Missour (origin Morocco) were superior to other rootstocks. The use of green cuttings shortened and simplified considerably the traditional rootstock preparation procedure. Various sizes of shoot-tips excised from almond varieties were grafted in vitro and regeneration rates were determined. About 50% graft success was obtained with shoottips consisting of a meristematic dome and three to four leaf primordia. 'STG plants' consisting of the in vitro rootstocks and developed shoot tips (almond variety), were side-grafted in the greenhouse onto vigorous GF305 plants. Rapid growth of the grafted STG plants was observed regardless of the season. Virus status was determined in side-grafted STG plants as soon as leaf material could be collected for an ELISA / PCR assay. Scions found free of the tested viruses were kept and following further development and repeated testing could be harvested for propagation. With these results, an improvement of the STG technique was obtained for almond sanitation. The study is ongoing and its application for other Prunus species will be attempted.
Scientific Publication
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