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NMAT4, A maturase factor required for nad1 pre-mRNA processing and maturation, is essential for holocomplex i biogenesis in Arabidopsis mitochondria
Year:
2014
Source of publication :
Plant Journal
Authors :
Belausov, Eduard
;
.
Mani, Yair
;
.
Volume :
78
Co-Authors:
Cohen, S., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
Zmudjak, M., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
Colas Des Francs-Small, C., Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Malik, S., Department of Biology, McGill University, Montreal, QC H3A 1B1, Canada
Shaya, F., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Keren, I., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel, Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Many, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Brown, G.G., Department of Biology, McGill University, Montreal, QC H3A 1B1, Canada
Small, I., Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Ostersetzer-Biran, O., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
Facilitators :
From page:
253
To page:
268
(
Total pages:
16
)
Abstract:
Group II introns are large catalytic RNAs that are found in bacteria and organellar genomes of lower eukaryotes, but are particularly prevalent within mitochondria in plants, where they are present in many critical genes. The excision of plant mitochondrial introns is essential for respiratory functions, and is facilitated in vivo by various protein cofactors. Typical group II introns are classified as mobile genetic elements, consisting of the self-splicing ribozyme and its own intron-encoded maturase protein. A hallmark of maturases is that they are intron-specific, acting as cofactors that bind their intron-containing pre-RNAs to facilitate splicing. However, the degeneracy of the mitochondrial introns in plants and the absence of cognate intron-encoded maturase open reading frames suggest that their splicing in vivo is assisted by 'trans'-acting protein factors. Interestingly, angiosperms harbor several nuclear-encoded maturase-related (nMat) genes that contain N-terminal mitochondrial localization signals. Recently, we established the roles of two of these paralogs in Arabidopsis, nMAT1 and nMAT2, in the splicing of mitochondrial introns. Here we show that nMAT4 (At1g74350) is required for RNA processing and maturation of nad1 introns 1, 3 and 4 in Arabidopsis mitochondria. Seed germination, seedling establishment and development are strongly affected in homozygous nmat4 mutants, which also show modified respiration phenotypes that are tightly associated with complex I defects. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.
Note:
Related Files :
arabidopsis
Genetics
germination
metabolism
Nucleic acids
phenotype
proteins
Show More
Related Content
More details
DOI :
10.1111/tpj.12466
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29992
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
NMAT4, A maturase factor required for nad1 pre-mRNA processing and maturation, is essential for holocomplex i biogenesis in Arabidopsis mitochondria
78
Cohen, S., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
Zmudjak, M., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
Colas Des Francs-Small, C., Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Malik, S., Department of Biology, McGill University, Montreal, QC H3A 1B1, Canada
Shaya, F., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Keren, I., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel, Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794-5215, United States
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Many, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Brown, G.G., Department of Biology, McGill University, Montreal, QC H3A 1B1, Canada
Small, I., Australian Research Council Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
Ostersetzer-Biran, O., Department of Plant and Environmental Sciences, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat-Ram Jerusalem 91904, Israel
NMAT4, A maturase factor required for nad1 pre-mRNA processing and maturation, is essential for holocomplex i biogenesis in Arabidopsis mitochondria
Group II introns are large catalytic RNAs that are found in bacteria and organellar genomes of lower eukaryotes, but are particularly prevalent within mitochondria in plants, where they are present in many critical genes. The excision of plant mitochondrial introns is essential for respiratory functions, and is facilitated in vivo by various protein cofactors. Typical group II introns are classified as mobile genetic elements, consisting of the self-splicing ribozyme and its own intron-encoded maturase protein. A hallmark of maturases is that they are intron-specific, acting as cofactors that bind their intron-containing pre-RNAs to facilitate splicing. However, the degeneracy of the mitochondrial introns in plants and the absence of cognate intron-encoded maturase open reading frames suggest that their splicing in vivo is assisted by 'trans'-acting protein factors. Interestingly, angiosperms harbor several nuclear-encoded maturase-related (nMat) genes that contain N-terminal mitochondrial localization signals. Recently, we established the roles of two of these paralogs in Arabidopsis, nMAT1 and nMAT2, in the splicing of mitochondrial introns. Here we show that nMAT4 (At1g74350) is required for RNA processing and maturation of nad1 introns 1, 3 and 4 in Arabidopsis mitochondria. Seed germination, seedling establishment and development are strongly affected in homozygous nmat4 mutants, which also show modified respiration phenotypes that are tightly associated with complex I defects. © 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.
Scientific Publication
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