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Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism
Year:
2006
Authors :
Paran, Ilan
;
.
Volume :
83
Co-Authors:
Brown, C.R., USDA/ARS, Prosser, WA, United States
Kim, T.S., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Ganga, Z., Aroostook Research Farm, University of Maine, Presque Isle, ME, United States
Haynes, K., USDA/ARS, Beltsville, MD, United States
De Jong, D., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Jahn, M., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Paran, I., Volcani Center, Bet Dagan, Israel
De Jong, W., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Facilitators :
From page:
365
To page:
372
(
Total pages:
8
)
Abstract:
High carotenoid potato may have particular value for human health due to the antioxidant properties and the therapeutic value for eye health in patients at risk for macular degeneration. Carotenoid concentrations were determined among the progeny of a cross between two high carotenoid lines derived from diploid Papa Amarilla germplasm from South America. The total carotenoid content ranged from 82 to 2686 μg / 100 g fresh weight (FW). The higher values greatly exceeded the mid-parent value of the cross. An index for yellow tuber flesh color was determined for a subset of the progeny. A cleaved amplified polymorphic sequence (CAPS) assay was developed to distinguish the alleles of beta-carotene hydroxylase (bch) in the two high-carotenoid parents. A bch allele (denoted B) common to the high carotenoid parents co-segregated with yellow flesh in the progeny of a white-flesh x yellow-flesh cross, making bch an excellent candidate for the classical Y locus, required for yellow tuber flesh. The same allele was also present in all other yellow-fleshed potato clones tested. Genotype at bch explained a portion of the variation of total carotenoid (R2 = 0.42). Clones homozygous for the B allele (BB) contained, on average, slightly more carotenoid than heterozygous Bb clones, which in turn had much more carotenoid than homozygous bb clones, suggesting a partially dominant gene model. Similarly, bb flesh was significantly less yellow than Bb and BB, the latter two being quite close. Total carotenoid varied considerably between progeny in the Bb and BB genotype categories, suggesting that variation at one or more additional loci have a significant effect on total carotenoid levels. Since the total carotenoid levels in many Papa Amarilla cultivars and progeny are much higher than those in white- and yellow-fleshed tetraploid cultivars, it may be possible to breed for high carotenoid values in tetraploid germplasm by introducing one or more genes derived from Papa Amarilla germplasm.
Note:
Related Files :
breeding
Goniocalyx
Papa Amarilla
Phureja
Solanum
Solanum tuberosum
Zeaxanthin
Show More
Related Content
More details
DOI :
10.1007/BF02872013
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
29996
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
Scientific Publication
Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism
83
Brown, C.R., USDA/ARS, Prosser, WA, United States
Kim, T.S., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Ganga, Z., Aroostook Research Farm, University of Maine, Presque Isle, ME, United States
Haynes, K., USDA/ARS, Beltsville, MD, United States
De Jong, D., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Jahn, M., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Paran, I., Volcani Center, Bet Dagan, Israel
De Jong, W., Dept. of Plant Breeding, Cornell University, Ithaca, NY, United States
Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism
High carotenoid potato may have particular value for human health due to the antioxidant properties and the therapeutic value for eye health in patients at risk for macular degeneration. Carotenoid concentrations were determined among the progeny of a cross between two high carotenoid lines derived from diploid Papa Amarilla germplasm from South America. The total carotenoid content ranged from 82 to 2686 μg / 100 g fresh weight (FW). The higher values greatly exceeded the mid-parent value of the cross. An index for yellow tuber flesh color was determined for a subset of the progeny. A cleaved amplified polymorphic sequence (CAPS) assay was developed to distinguish the alleles of beta-carotene hydroxylase (bch) in the two high-carotenoid parents. A bch allele (denoted B) common to the high carotenoid parents co-segregated with yellow flesh in the progeny of a white-flesh x yellow-flesh cross, making bch an excellent candidate for the classical Y locus, required for yellow tuber flesh. The same allele was also present in all other yellow-fleshed potato clones tested. Genotype at bch explained a portion of the variation of total carotenoid (R2 = 0.42). Clones homozygous for the B allele (BB) contained, on average, slightly more carotenoid than heterozygous Bb clones, which in turn had much more carotenoid than homozygous bb clones, suggesting a partially dominant gene model. Similarly, bb flesh was significantly less yellow than Bb and BB, the latter two being quite close. Total carotenoid varied considerably between progeny in the Bb and BB genotype categories, suggesting that variation at one or more additional loci have a significant effect on total carotenoid levels. Since the total carotenoid levels in many Papa Amarilla cultivars and progeny are much higher than those in white- and yellow-fleshed tetraploid cultivars, it may be possible to breed for high carotenoid values in tetraploid germplasm by introducing one or more genes derived from Papa Amarilla germplasm.
Scientific Publication
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