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Role of cuticular lipids and water-soluble compounds in tick susceptibility to Metarhizium infection
Year:
2013
Source of publication :
Biocontrol Science and Technology
Authors :
Eshel, Dani
;
.
Gindin, Galina
;
.
Glazer, Itamar
;
.
Ment, Dana
;
.
Samish, Michael
;
.
Teper-Bamnolker, Paula
;
.
Volume :
23
Co-Authors:
Ment, D., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Gindin, G., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Rot, A., Association for Research in Otolaryngology (ARO), Kimron Veterinary Institute, Bet-Dagan, Israel
Eshel, D., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Teper-Bamnolker, P., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Ben-Ze'ev, I., Plant Protection and Inspection Services, Ministry of Agriculture and Rural Development, Bet Dagan Agricultural Center, Bet Dagan, Israel
Glazer, I., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Samish, M., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Facilitators :
From page:
956
To page:
967
(
Total pages:
12
)
Abstract:
The arthropod cuticle acts as a physiochemical barrier protecting the organism from pathogens' entry. Entomopathogenic fungi actively penetrate the cuticles of arthropod hosts and are therefore directly affected by cuticle composition. Previously we have observed that Metarhizium spp. developing on resistant ticks ultimately die without penetrating tick's cuticle, suggesting that the cuticles of resistant ticks have antifungal compounds. In the present study, lipids and water-soluble cuticular components were extracted from engorged female tick cuticles, of one susceptible and one resistant tick species to Metarhizium spp. While conidia exposed to lipids from the susceptible tick, Rhipicephalus annulatus, germinated and differentiated into appressorium, conidia exposed to lipids from the resistant tick, Hyalomma excavatum, were inhibited. Soluble cuticular component extracts from both susceptible and resistant ticks stimulated conidial germination but not appressorium differentiation. A comparative analysis of the fatty acid profile in lipid extract of each tick exhibited similar compositions, but the relative abundance of C16:0, C18:0, C18:1ω9C and C20:0 was 2-5 times higher in the extracts from resistant ticks. All of these fatty acids inhibited conidial germination in vitro at 1% and 0.1% w/v concentration, but C20:0 stimulated appressorium differentiation at low concentration. This is the first report demonstrating a possible link between the presence of antifungal compounds in a specific concentration in tick cuticle and tick resistance to infection. © 2013 Copyright Taylor and Francis Group, LLC.
Note:
Related Files :
appressorium
biochemical composition
germination
Metarhizium
soluble cuticular components
tick
Show More
Related Content
More details
DOI :
10.1080/09583157.2013.811217
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30005
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Role of cuticular lipids and water-soluble compounds in tick susceptibility to Metarhizium infection
23
Ment, D., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
Gindin, G., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Rot, A., Association for Research in Otolaryngology (ARO), Kimron Veterinary Institute, Bet-Dagan, Israel
Eshel, D., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Teper-Bamnolker, P., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Ben-Ze'ev, I., Plant Protection and Inspection Services, Ministry of Agriculture and Rural Development, Bet Dagan Agricultural Center, Bet Dagan, Israel
Glazer, I., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Samish, M., Association for Research in Otolaryngology (ARO), The Volcani Center, Bet-Dagan, Israel
Role of cuticular lipids and water-soluble compounds in tick susceptibility to Metarhizium infection
The arthropod cuticle acts as a physiochemical barrier protecting the organism from pathogens' entry. Entomopathogenic fungi actively penetrate the cuticles of arthropod hosts and are therefore directly affected by cuticle composition. Previously we have observed that Metarhizium spp. developing on resistant ticks ultimately die without penetrating tick's cuticle, suggesting that the cuticles of resistant ticks have antifungal compounds. In the present study, lipids and water-soluble cuticular components were extracted from engorged female tick cuticles, of one susceptible and one resistant tick species to Metarhizium spp. While conidia exposed to lipids from the susceptible tick, Rhipicephalus annulatus, germinated and differentiated into appressorium, conidia exposed to lipids from the resistant tick, Hyalomma excavatum, were inhibited. Soluble cuticular component extracts from both susceptible and resistant ticks stimulated conidial germination but not appressorium differentiation. A comparative analysis of the fatty acid profile in lipid extract of each tick exhibited similar compositions, but the relative abundance of C16:0, C18:0, C18:1ω9C and C20:0 was 2-5 times higher in the extracts from resistant ticks. All of these fatty acids inhibited conidial germination in vitro at 1% and 0.1% w/v concentration, but C20:0 stimulated appressorium differentiation at low concentration. This is the first report demonstrating a possible link between the presence of antifungal compounds in a specific concentration in tick cuticle and tick resistance to infection. © 2013 Copyright Taylor and Francis Group, LLC.
Scientific Publication
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