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The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from Siraitia grosvenorii
Year:
2016
Authors :
Baranes, Nadine
;
.
Burger, Joseph
;
.
Cohen, Shahar
;
.
Davidovich-Rikanati, Rachel
;
.
Doron-Faigenboim, Adi
;
.
Freilich, Shiri
;
.
Itkin, Maxim
;
.
Katzir, Nurit
;
.
Lewinsohn, Efraim
;
.
Oren, Elad
;
.
Paris, Harry
;
.
Petreikov, Marina
;
.
Portnoy, Vitaly H.
;
.
Schaffer, Arthur
;
.
Shen, Shmuel
;
.
Tadmor, Yaakov
;
.
Tzuri, Galil
;
.
Volume :
113
Co-Authors:
Itkin, M., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Davidovich-Rikanati, R., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Cohen, S., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Portnoy, V., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Doron-Faigenboim, A., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Oren, E., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Freilich, S., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Tzuri, G., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Baranes, N., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Shen, S., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Petreikov, M., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Sertchook, R., Protein Modelling, Gedera, Israel
Ben-Dor, S., Department of Biological Services, Weizmann Institute of Science, Rehovot, Israel
Gottlieb, H., Department of Chemistry, Bar-Ilan University, Qiryat Ono, Israel
Hernandez, A., Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, United States
Nelson, D.R., Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States
Paris, H.S., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Tadmor, Y., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Burger, Y., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Lewinsohn, E., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Katzir, N., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Schaffer, A., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
7619
To page:
7628
(
Total pages:
10
)
Abstract:
The consumption of sweeteners, natural as well as synthetic sugars, is implicated in an array of modern-day health problems. Therefore, natural nonsugar sweeteners are of increasing interest. We identify here the biosynthetic pathway of the sweet triterpenoid glycoside mogroside V, which has a sweetening strength of 250 times that of sucrose and is derived from mature fruit of luohanguo (Siraitia grosvenorii, monk fruit). A whole-genome sequencing of Siraitia, leading to a preliminary draft of the genome, was combined with an extensive transcriptomic analysis of developing fruit. A functional expression survey of nearly 200 candidate genes identified the members of the five enzyme families responsible for the synthesis of mogroside V: squalene epoxidases, triterpenoid synthases, epoxide hydrolases, cytochrome P450s, and UDP-glucosyltransferases. Protein modeling and docking studies corroborated the experimentally proven functional enzyme activities and indicated the order of the metabolic steps in the pathway. A comparison of the genomic organization and expression patterns of these Siraitia genes with the orthologs of other Cucurbitaceae implicates a strikingly coordinated expression of the pathway in the evolution of this speciesspecific and valuable metabolic pathway. The genomic organization of the pathway genes, syntenously preserved among the Cucurbitaceae, indicates, on the other hand, that gene clustering cannot account for this novel secondary metabolic pathway.
Note:

See correction at:

http://www.pnas.org/content/115/16/E3862

Related Files :
Conference paper
Cucurbitaceae
gene expression
metabolism
Metabolomics
Molecular Evolution
Plant Genome
Show More
Related Content
More details
DOI :
10.1073/pnas.1604828113
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
30008
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
You may also be interested in
Scientific Publication
The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from Siraitia grosvenorii
113
Itkin, M., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Davidovich-Rikanati, R., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Cohen, S., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Portnoy, V., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Doron-Faigenboim, A., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Oren, E., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Freilich, S., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Tzuri, G., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Baranes, N., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Shen, S., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Petreikov, M., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
Sertchook, R., Protein Modelling, Gedera, Israel
Ben-Dor, S., Department of Biological Services, Weizmann Institute of Science, Rehovot, Israel
Gottlieb, H., Department of Chemistry, Bar-Ilan University, Qiryat Ono, Israel
Hernandez, A., Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, United States
Nelson, D.R., Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, United States
Paris, H.S., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Tadmor, Y., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Burger, Y., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Lewinsohn, E., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Katzir, N., Institute of Plant Sciences, Agricultural Research Organization-Newe Ya'ar Center, Ramat Yishay, Israel
Schaffer, A., Institute of Plant Sciences, Agricultural Research Organization-Volcani Center, Bet Dagan, Israel
The biosynthetic pathway of the nonsugar, high-intensity sweetener mogroside V from Siraitia grosvenorii
The consumption of sweeteners, natural as well as synthetic sugars, is implicated in an array of modern-day health problems. Therefore, natural nonsugar sweeteners are of increasing interest. We identify here the biosynthetic pathway of the sweet triterpenoid glycoside mogroside V, which has a sweetening strength of 250 times that of sucrose and is derived from mature fruit of luohanguo (Siraitia grosvenorii, monk fruit). A whole-genome sequencing of Siraitia, leading to a preliminary draft of the genome, was combined with an extensive transcriptomic analysis of developing fruit. A functional expression survey of nearly 200 candidate genes identified the members of the five enzyme families responsible for the synthesis of mogroside V: squalene epoxidases, triterpenoid synthases, epoxide hydrolases, cytochrome P450s, and UDP-glucosyltransferases. Protein modeling and docking studies corroborated the experimentally proven functional enzyme activities and indicated the order of the metabolic steps in the pathway. A comparison of the genomic organization and expression patterns of these Siraitia genes with the orthologs of other Cucurbitaceae implicates a strikingly coordinated expression of the pathway in the evolution of this speciesspecific and valuable metabolic pathway. The genomic organization of the pathway genes, syntenously preserved among the Cucurbitaceae, indicates, on the other hand, that gene clustering cannot account for this novel secondary metabolic pathway.

See correction at:

http://www.pnas.org/content/115/16/E3862

Scientific Publication
You may also be interested in