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Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina
Year:
2002
Source of publication :
Plant physiology (source)
Authors :
Sadka, Avi
;
.
Volume :
129
Co-Authors:
Azachi, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Sadka, A., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Fisher, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Goldshlag, P., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Gokhman, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Zamir, A., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
1320
To page:
1329
(
Total pages:
10
)
Abstract:
In studies of the outstanding salt tolerance of the unicellular green alga Dunaliella salina, we isolated a cDNA for a salt-inducible mRNA encoding a protein homologous to plant β-ketoacyl-coenzyme A (CoA) synthases (Kcs). These microsomal enzymes catalyze the condensation of malonyl-CoA with acyl-CoA, the first and rate-limiting step in fatty acid elongation. Kcs activity, localized to a D. salina microsomal fraction, increased in cells transferred from 0.5 to 3.5 M NaCl, as did the level of the kcs mRNA. The function of the kcs gene product was directly demonstrated by the condensing activity exhibited by Escherichia coli cells expressing the kcs cDNA. The effect of salinity on kcs expression in D. salina suggested the possibility that salt adaptation entailed modifications in the fatty acid composition of algal membranes. Lipid analyses indicated that microsomes, but not plasma membranes or thylakoids, from cells grown in 3.5 M NaCl contained a considerably higher ratio of C18 (mostly unsaturated) to C16 (mostly saturated) fatty acids compared with cells grown in 0.5 M salt. Thus, the salt-inducible Kcs, jointly with fatty acid desaturases, may play a role in adapting intracellular membrane compartments to function in the high internal glycerol concentrations balancing the external osmotic pressure.
Note:
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More details
DOI :
10.1104/pp.001909
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30011
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina
129
Azachi, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Sadka, A., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Fisher, M., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Goldshlag, P., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Gokhman, I., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Zamir, A., Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Salt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina
In studies of the outstanding salt tolerance of the unicellular green alga Dunaliella salina, we isolated a cDNA for a salt-inducible mRNA encoding a protein homologous to plant β-ketoacyl-coenzyme A (CoA) synthases (Kcs). These microsomal enzymes catalyze the condensation of malonyl-CoA with acyl-CoA, the first and rate-limiting step in fatty acid elongation. Kcs activity, localized to a D. salina microsomal fraction, increased in cells transferred from 0.5 to 3.5 M NaCl, as did the level of the kcs mRNA. The function of the kcs gene product was directly demonstrated by the condensing activity exhibited by Escherichia coli cells expressing the kcs cDNA. The effect of salinity on kcs expression in D. salina suggested the possibility that salt adaptation entailed modifications in the fatty acid composition of algal membranes. Lipid analyses indicated that microsomes, but not plasma membranes or thylakoids, from cells grown in 3.5 M NaCl contained a considerably higher ratio of C18 (mostly unsaturated) to C16 (mostly saturated) fatty acids compared with cells grown in 0.5 M salt. Thus, the salt-inducible Kcs, jointly with fatty acid desaturases, may play a role in adapting intracellular membrane compartments to function in the high internal glycerol concentrations balancing the external osmotic pressure.
Scientific Publication
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