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Down-regulation of lactogenic hormone receptors in Nb2 lymphoma cells by cholera toxin
Year:
1987
Source of publication :
Biochemistry International
Authors :
Pines, Mark
;
.
Volume :
14
Co-Authors:
Ashkenazi, A., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Pines, M., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Gertler, A., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Facilitators :
From page:
1065
To page:
1072
(
Total pages:
8
)
Abstract:
Exposure of lactogen-dependent (Nb2-11C) and lactogen independent (Nb2-SP) lymphoma cells to cholera toxin (0.05-50 pM) resulted within 18-28 h in a 50% decrease in the binding capacity of the intact cells to iodinated human growth hormone, and 40% decrease in cell-homogenates. Scatchard analysis revealed that the reduction in binding resulted from loss of cell-surface receptors accompanied by degradation of intracellular receptors. No alterations in receptor binding affinity were observed. One to 3 h of exposure to the toxin was sufficient to reduce the binding to the level obtained after continuous incubation with the toxin for 28 h. Addition of dibutyryl cAMP (0.1mM) to the medium resulted in similar down-regulation of lactogenic receptors.
Note:
Related Files :
Animal
cholera toxin
Cyclic AMP
drug effect
metabolism
prolactin receptor
Receptors, Prolactin
time
Tumor Cells, Cultured
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30014
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Down-regulation of lactogenic hormone receptors in Nb2 lymphoma cells by cholera toxin
14
Ashkenazi, A., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Pines, M., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Gertler, A., Department of Agricultural Biochemistry, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Down-regulation of lactogenic hormone receptors in Nb2 lymphoma cells by cholera toxin
Exposure of lactogen-dependent (Nb2-11C) and lactogen independent (Nb2-SP) lymphoma cells to cholera toxin (0.05-50 pM) resulted within 18-28 h in a 50% decrease in the binding capacity of the intact cells to iodinated human growth hormone, and 40% decrease in cell-homogenates. Scatchard analysis revealed that the reduction in binding resulted from loss of cell-surface receptors accompanied by degradation of intracellular receptors. No alterations in receptor binding affinity were observed. One to 3 h of exposure to the toxin was sufficient to reduce the binding to the level obtained after continuous incubation with the toxin for 28 h. Addition of dibutyryl cAMP (0.1mM) to the medium resulted in similar down-regulation of lactogenic receptors.
Scientific Publication
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