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Molecular tagging of the tobacco chromosome carrying the TMV-resistance gene (N gene) by Agrobacterium-mediated transformation
Year:
1992
Source of publication :
Theoretical and Applied Genetics
Authors :
Zelcer, Aaron
;
.
Volume :
83
Co-Authors:
Bates, G.W., Department of Biological Science B-165, Florida State University, Tallahassee, 32306-3015, FL, United States
Zelcer, A., Department of Plant Genetics and Breeding, A.R.O. The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
981
To page:
986
(
Total pages:
6
)
Abstract:
The hypersensitive response of tobacco to inoculation with tobacco mosaic virus (TMV) is controlled by a single dominant gene, the N gene. As a first step in localizing and transferring the N gene, we have prepared a line of tobacco plants in which the kanamycin-resistance (Kmr) gene is closely linked to the N gene. Nicotiana tabacum plants heterozygous for the N gene were transformed to Kmr by Agrobacterium carrying pMON200. Eighty-nine independent transformed clones were regenerated and were backcrossed with nontransformed, TMV-sensitive plants. Progeny from these crosses were screened first for Kmr; then the Kmr progeny were inoculated with TMV and scored for the hypersensitive response. Of the initial 89 clones, 68 appeared to have integrated a single functional Kmr gene. Initial tests for TMV resistance indicated possible linkage between Kmr and the N gene in 11 plants. With further testing, linkage has been established for two of these plant lines. In one of these lines, the two genes were 30-40 map units apart, and evidence of somatic instability in the linkage was obtained. However, in the second line, linkage between Kmr and the N gene was tight, and recombination between the genes in this case was only 5%. Southern hybridization revealed that this plant contained only a single copy of the Kmr gene. Linkage between Kmr and the N gene in this plant line has been verified in each of two additional backcross generations. © 1992 Springer-Verlag.
Note:
Related Files :
Hypersensitive response
Kanamycin Resistance
Nicotiana tabacum
Tobacco mosaic virus
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Related Content
More details
DOI :
10.1007/BF00232960
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30022
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Molecular tagging of the tobacco chromosome carrying the TMV-resistance gene (N gene) by Agrobacterium-mediated transformation
83
Bates, G.W., Department of Biological Science B-165, Florida State University, Tallahassee, 32306-3015, FL, United States
Zelcer, A., Department of Plant Genetics and Breeding, A.R.O. The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Molecular tagging of the tobacco chromosome carrying the TMV-resistance gene (N gene) by Agrobacterium-mediated transformation
The hypersensitive response of tobacco to inoculation with tobacco mosaic virus (TMV) is controlled by a single dominant gene, the N gene. As a first step in localizing and transferring the N gene, we have prepared a line of tobacco plants in which the kanamycin-resistance (Kmr) gene is closely linked to the N gene. Nicotiana tabacum plants heterozygous for the N gene were transformed to Kmr by Agrobacterium carrying pMON200. Eighty-nine independent transformed clones were regenerated and were backcrossed with nontransformed, TMV-sensitive plants. Progeny from these crosses were screened first for Kmr; then the Kmr progeny were inoculated with TMV and scored for the hypersensitive response. Of the initial 89 clones, 68 appeared to have integrated a single functional Kmr gene. Initial tests for TMV resistance indicated possible linkage between Kmr and the N gene in 11 plants. With further testing, linkage has been established for two of these plant lines. In one of these lines, the two genes were 30-40 map units apart, and evidence of somatic instability in the linkage was obtained. However, in the second line, linkage between Kmr and the N gene was tight, and recombination between the genes in this case was only 5%. Southern hybridization revealed that this plant contained only a single copy of the Kmr gene. Linkage between Kmr and the N gene in this plant line has been verified in each of two additional backcross generations. © 1992 Springer-Verlag.
Scientific Publication
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