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Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi, a wild peanut
Year:
2013
Source of publication :
PLoS ONE
Authors :
Kumar, Dilip
;
.
Volume :
8
Co-Authors:
Singh, N.K., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Kumar, K.R.R., Department of Plant Sciences, University of Hyderabad, Hyderabad, India, Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden
Kumar, D., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Shukla, P., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Kirti, P.B., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Facilitators :
From page:
To page:
(
Total pages:
1
)
Abstract:
Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi , a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants. © 2013 Singh et al.
Note:
Related Files :
Arachis hypogaea
Base Sequence
gene expression
genetic engineering
quantitative study
Rhizoctonia
salt stress
unclassified drug
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More details
DOI :
10.1371/journal.pone.0083963
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30031
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi, a wild peanut
8
Singh, N.K., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Kumar, K.R.R., Department of Plant Sciences, University of Hyderabad, Hyderabad, India, Department of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden
Kumar, D., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Shukla, P., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Kirti, P.B., Department of Plant Sciences, University of Hyderabad, Hyderabad, India
Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi, a wild peanut
Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi , a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants. © 2013 Singh et al.
Scientific Publication
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