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Spinach SoHXK1 is a mitochondria-associated hexokinase
Year:
2007
Source of publication :
Planta
Authors :
Belausov, Eduard
;
.
Damari-Weissler, Hila
;
.
Gidoni, David
;
.
Ginzburg, Alexandra
;
.
Granot, David
;
.
Mett, Anahit
;
.
Volume :
226
Co-Authors:
Damari-Weissler, H., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ginzburg, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gidoni, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mett, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Krassovskaya, I., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Weber, A.P.M., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1053
To page:
1058
(
Total pages:
6
)
Abstract:
Hexokinase, a hexose-phosphorylating enzyme, has emerged as a central enzyme in sugar-sensing processes. A few HXK isozymes have been identified in various plant species. These isozymes have been classified into two major groups; plastidic (type A) isozymes located in the plastid stroma and those containing a membrane anchor domain (type B) located mainly adjacent to the mitochondria, but also found in the nucleus. Of all the hexokinases that have been characterized to date, the only exception to this rule is a spinach type B HXK (SoHXK1) that, by means of subcellular fractionation, has been localized to the outer membrane of plastids. However, SoHXK1 has a membrane anchor domain that is almost identical to that of the other type B HXKs. To determine the localization of SoHXK1 enzyme by other means, we expressed SoHXK1::GFP fusion protein in tobacco and Arabidopsis protoplasts and compared its localization with that of the Arabidopsis AtHXK1::GFP fusion protein that shares a similar N-terminal membrane anchor domain. SoHXK1::GFP is localized adjacent to the mitochondria, similar to AtHXK1::GFP and all other previously examined type B HXKs. Proteomic analysis had previously identified AtHXK1 on the outside of the mitochondrial membrane. We, therefore, suggest that SoHXK1 enzyme is located adjacent to the mitochondria like the other type B HXKs that share the same N-terminal membrane anchor domain. © 2007 Springer-Verlag.
Note:
Related Files :
arabidopsis
gene expression
Genetics
Hexose phosphorylation
metabolism
molecular genetics
spinach
Spinacia oleracea
Show More
Related Content
More details
DOI :
10.1007/s00425-007-0546-7
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30088
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
You may also be interested in
Scientific Publication
Spinach SoHXK1 is a mitochondria-associated hexokinase
226
Damari-Weissler, H., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Ginzburg, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gidoni, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mett, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Krassovskaya, I., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Weber, A.P.M., Department of Plant Biology, Michigan State University, East Lansing, MI 48824, United States
Belausov, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Spinach SoHXK1 is a mitochondria-associated hexokinase
Hexokinase, a hexose-phosphorylating enzyme, has emerged as a central enzyme in sugar-sensing processes. A few HXK isozymes have been identified in various plant species. These isozymes have been classified into two major groups; plastidic (type A) isozymes located in the plastid stroma and those containing a membrane anchor domain (type B) located mainly adjacent to the mitochondria, but also found in the nucleus. Of all the hexokinases that have been characterized to date, the only exception to this rule is a spinach type B HXK (SoHXK1) that, by means of subcellular fractionation, has been localized to the outer membrane of plastids. However, SoHXK1 has a membrane anchor domain that is almost identical to that of the other type B HXKs. To determine the localization of SoHXK1 enzyme by other means, we expressed SoHXK1::GFP fusion protein in tobacco and Arabidopsis protoplasts and compared its localization with that of the Arabidopsis AtHXK1::GFP fusion protein that shares a similar N-terminal membrane anchor domain. SoHXK1::GFP is localized adjacent to the mitochondria, similar to AtHXK1::GFP and all other previously examined type B HXKs. Proteomic analysis had previously identified AtHXK1 on the outside of the mitochondrial membrane. We, therefore, suggest that SoHXK1 enzyme is located adjacent to the mitochondria like the other type B HXKs that share the same N-terminal membrane anchor domain. © 2007 Springer-Verlag.
Scientific Publication
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