נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Antibody response to Hepatozoon canis in experimentally infected dogs
Year:
1998
Source of publication :
Veterinary Parasitology
Authors :
Samish, Michael
;
.
Volume :
74
Co-Authors:
Baneth, G., School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel
Shkap, V., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Pipano, E., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Savitsky, I., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Facilitators :
From page:
299
To page:
305
(
Total pages:
7
)
Abstract:
Canine hepatozoonosis is a disease caused by the tick-borne protozoan Hepatozoon canis. Five puppies were inoculated by ingestion of Rhipicephalus sanguineus ticks experimentally infected with H. canis, and all became infected with H. canis: gametocytes were detected in blood smears from four dogs and schizonts were observed in the spleen and bone marrow of the fifth. Antibodies reactive with H. canis gametocytes were detected by the indirect fluorescent antibody test (IFA), with IgM detected initially in all dogs 16 to 39 days post infection (PI) and IgG 22 to 43 days PI. The presence of gametocytes was first observed within peripheral blood neutrophils in Giemsa- stained blood smears between days 28 and 43 PI. Gametocyte-reactive antibodies were detected before the appearance of blood gametocytes in three of the four parasitemic dogs and also in a dog with no observed parasitemia. The detection of serum antibodies prior to the detection of blood gametocytes, or without apparent parasitemia, suggests that antibodies reactive with gametocytes may be formed against earlier forms of the parasite developing in the parenchymal tissues. Sera of dogs experimentally infected with Babesia canis, Babesia gibsoni and Ehrlichia canis exhibited no reactivity when tested with H. canis antigen. Additionally, sera positive for H. canis were not reactive with antigens of Toxoplasma gondii, Neospora caninum, Leishmania donovani and E. canis. In conclusion, inoculation of dogs with ticks infected with H. canis results in production of antibodies reactive with peripheral blood gametocytes. Detection of IgG titres would be beneficial for the diagnosis of progressive infections with undetectable parasitemia, for seroprevalence studies, and as an adjunct to IgM titres in early infections.
Note:
Related Files :
Acari
animal experiment
animal model
Animals
animal tissue
immunoglobulin G
protozoal infection
ticks
Show More
Related Content
More details
DOI :
10.1016/S0304-4017(97)00160-X
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30103
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
You may also be interested in
Scientific Publication
Antibody response to Hepatozoon canis in experimentally infected dogs
74
Baneth, G., School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel
Shkap, V., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Samish, M., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Pipano, E., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Savitsky, I., Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
Antibody response to Hepatozoon canis in experimentally infected dogs
Canine hepatozoonosis is a disease caused by the tick-borne protozoan Hepatozoon canis. Five puppies were inoculated by ingestion of Rhipicephalus sanguineus ticks experimentally infected with H. canis, and all became infected with H. canis: gametocytes were detected in blood smears from four dogs and schizonts were observed in the spleen and bone marrow of the fifth. Antibodies reactive with H. canis gametocytes were detected by the indirect fluorescent antibody test (IFA), with IgM detected initially in all dogs 16 to 39 days post infection (PI) and IgG 22 to 43 days PI. The presence of gametocytes was first observed within peripheral blood neutrophils in Giemsa- stained blood smears between days 28 and 43 PI. Gametocyte-reactive antibodies were detected before the appearance of blood gametocytes in three of the four parasitemic dogs and also in a dog with no observed parasitemia. The detection of serum antibodies prior to the detection of blood gametocytes, or without apparent parasitemia, suggests that antibodies reactive with gametocytes may be formed against earlier forms of the parasite developing in the parenchymal tissues. Sera of dogs experimentally infected with Babesia canis, Babesia gibsoni and Ehrlichia canis exhibited no reactivity when tested with H. canis antigen. Additionally, sera positive for H. canis were not reactive with antigens of Toxoplasma gondii, Neospora caninum, Leishmania donovani and E. canis. In conclusion, inoculation of dogs with ticks infected with H. canis results in production of antibodies reactive with peripheral blood gametocytes. Detection of IgG titres would be beneficial for the diagnosis of progressive infections with undetectable parasitemia, for seroprevalence studies, and as an adjunct to IgM titres in early infections.
Scientific Publication
You may also be interested in