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Isolation of a fruit weight 2.2 (fw2.2) homolog in 'hass' avocado and characterization of its expression as related to reduced cell division and small fruit phenotype
Year:
2010
Source of publication :
Acta Horticulturae
Authors :
Dahan, Yardena
;
.
Irihimovitch, Vered
;
.
Rosenfeld, Revital
;
.
Volume :
884
Co-Authors:
Dahan, Y., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Rosenfeld, R., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Irihimovitch, V., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Facilitators :
From page:
145
To page:
152
(
Total pages:
8
)
Abstract:
The 'Hass' avocado (Persea americana Mill.) cultivar dominates the world avocado industry. Despite its popularity, this cultivar is known to be problematic in terms of fruit size. 'Hass' trees produce two populations of fruits: normal (NF) and phenotypically small (SF), A high proportion of the latter are produced even under favorable conditions, contributing to economic losses. SF growth is limited by cell number and not by cell size, indicating that events controlling cell division play a key role in fruit-size determination. In tomato, FRUIT WEIGHT 2.2 (FW2.2) is one of several QTLs controlling fruit size. Its effect is caused by a single gene (Lefw2.2) encoding a transmembrane protein proposed to function as a negative regulator of fruit cell division. In this study, full-length cDNA (Pafw2.2) encoding a FW2.2-like protein was isolated from 'Hass' SF mesocarp tissue. The identified Pafw2.2 encodes a predicted 180-aa protein with two transmembrane domains, and shares 62% identity with Lefw2.2. Semi-quantitative RT-PCR analysis revealed that throughout avocado fruit growth, the transcript level of Pafw2.2 was markedly higher in various SF tissues than in those same tissues in NF. Remarkably, in seeds, Pafw2.2 transcript accumulation was inversely correlated with transcript accumulation of the cellproliferation- related genes PaPCNA, and PaCycA1. Moreover, during fruit development, both mesocarp and seed cell numbers were significantly higher in NF, yet there were no significant differences in cell area between NF and SF. Taken together, our correlative data suggest that in avocado, Pafw2.2 may function similarly to Lefw2.2 by negatively regulating fruit cell division. Further exploration of Pafw2.2 function is therefore warranted. A better understanding of factors regulating fruit cell-division activity is the key to understanding processes affecting avocado fruit retention and size, and could eventually lead to the establishment of improved plant growth regulator (PGR) treatments to increase 'Hass' yield.
Note:
Related Files :
Cell-proliferation-related genes
Lycopersicon esculentum
Normal fruit (NF)
Persea americana
Persea americana Mill.
Phenotypically small fruit (SF)
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Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
30106
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Isolation of a fruit weight 2.2 (fw2.2) homolog in 'hass' avocado and characterization of its expression as related to reduced cell division and small fruit phenotype
884
Dahan, Y., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Rosenfeld, R., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Irihimovitch, V., Volcani Center, Horticulture Department, Bet-Dagan, Israel
Isolation of a fruit weight 2.2 (fw2.2) homolog in 'hass' avocado and characterization of its expression as related to reduced cell division and small fruit phenotype
The 'Hass' avocado (Persea americana Mill.) cultivar dominates the world avocado industry. Despite its popularity, this cultivar is known to be problematic in terms of fruit size. 'Hass' trees produce two populations of fruits: normal (NF) and phenotypically small (SF), A high proportion of the latter are produced even under favorable conditions, contributing to economic losses. SF growth is limited by cell number and not by cell size, indicating that events controlling cell division play a key role in fruit-size determination. In tomato, FRUIT WEIGHT 2.2 (FW2.2) is one of several QTLs controlling fruit size. Its effect is caused by a single gene (Lefw2.2) encoding a transmembrane protein proposed to function as a negative regulator of fruit cell division. In this study, full-length cDNA (Pafw2.2) encoding a FW2.2-like protein was isolated from 'Hass' SF mesocarp tissue. The identified Pafw2.2 encodes a predicted 180-aa protein with two transmembrane domains, and shares 62% identity with Lefw2.2. Semi-quantitative RT-PCR analysis revealed that throughout avocado fruit growth, the transcript level of Pafw2.2 was markedly higher in various SF tissues than in those same tissues in NF. Remarkably, in seeds, Pafw2.2 transcript accumulation was inversely correlated with transcript accumulation of the cellproliferation- related genes PaPCNA, and PaCycA1. Moreover, during fruit development, both mesocarp and seed cell numbers were significantly higher in NF, yet there were no significant differences in cell area between NF and SF. Taken together, our correlative data suggest that in avocado, Pafw2.2 may function similarly to Lefw2.2 by negatively regulating fruit cell division. Further exploration of Pafw2.2 function is therefore warranted. A better understanding of factors regulating fruit cell-division activity is the key to understanding processes affecting avocado fruit retention and size, and could eventually lead to the establishment of improved plant growth regulator (PGR) treatments to increase 'Hass' yield.
Scientific Publication
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