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Effect of oxazaborolidines on immobilized fructosyltransferase analyzed by surface plasmon resonance
Year:
2007
Source of publication :
Biosensors and Bioelectronics
Authors :
Shemesh, Moshe
;
.
Volume :
22
Co-Authors:
Jabbour, A., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel, Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Hebrew University, Jerusalem, 91120, Israel
Shemesh, M., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Srebnik, M., Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Hebrew University, Jerusalem, 91120, Israel
Zaks, B., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Steinberg, D., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Facilitators :
From page:
1658
To page:
1663
(
Total pages:
6
)
Abstract:
Dental diseases are among the most prevalent afflictions of humankind. These diseases are associated with the formation of biofilms harboring pathogenic bacteria. Fructosyltransferases (FTF) are extra cellular enzymes of several oral bacteria. FTF are associated with the formation of extracellular polysaccharide matrix (fructans) which play a role in biofilm formation and oral bacteria physiology. Oxazaborolidines have been shown to inhibit biofilm formation. The purpose of this study was to examine if the anti-biofilm effect is, in part, an effect on the immobilized enzymes synthesizing the extra cellular polysaccharide participating in biofilm formation. Eight different oxazaborolidines (BNO1-BNO8) were synthesized and evaluated for their affect on the synthesis of fructans by FTF using the biomolecular interaction analysis (BIAcore) system which involves the use of real-time surface plasmon resonance (SPR) technique. The tested oxazaborolidines demonstrated a significant and immediate inhibitory effect on immobilized FTF activity. This effect was reversible. Our results show that oxazaborolidines can act as enzymatic inhibitors of FTF immobilized on the surface, also at levels lower than their MIC. Part of the anti-biofilm effect of BNOs may be accounted for this enzymatic inhibition. © 2006.
Note:
Related Files :
bacteria
Dental prostheses
drug effect
Enzyme immobilization
immobilized enzyme
Polysaccharides
Show More
Related Content
More details
DOI :
10.1016/j.bios.2006.07.029
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30114
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
You may also be interested in
Scientific Publication
Effect of oxazaborolidines on immobilized fructosyltransferase analyzed by surface plasmon resonance
22
Jabbour, A., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel, Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Hebrew University, Jerusalem, 91120, Israel
Shemesh, M., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Srebnik, M., Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Hebrew University, Jerusalem, 91120, Israel
Zaks, B., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Steinberg, D., Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, POB 12272, Jerusalem, 91120, Israel
Effect of oxazaborolidines on immobilized fructosyltransferase analyzed by surface plasmon resonance
Dental diseases are among the most prevalent afflictions of humankind. These diseases are associated with the formation of biofilms harboring pathogenic bacteria. Fructosyltransferases (FTF) are extra cellular enzymes of several oral bacteria. FTF are associated with the formation of extracellular polysaccharide matrix (fructans) which play a role in biofilm formation and oral bacteria physiology. Oxazaborolidines have been shown to inhibit biofilm formation. The purpose of this study was to examine if the anti-biofilm effect is, in part, an effect on the immobilized enzymes synthesizing the extra cellular polysaccharide participating in biofilm formation. Eight different oxazaborolidines (BNO1-BNO8) were synthesized and evaluated for their affect on the synthesis of fructans by FTF using the biomolecular interaction analysis (BIAcore) system which involves the use of real-time surface plasmon resonance (SPR) technique. The tested oxazaborolidines demonstrated a significant and immediate inhibitory effect on immobilized FTF activity. This effect was reversible. Our results show that oxazaborolidines can act as enzymatic inhibitors of FTF immobilized on the surface, also at levels lower than their MIC. Part of the anti-biofilm effect of BNOs may be accounted for this enzymatic inhibition. © 2006.
Scientific Publication
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