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Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e
Year:
1974
Source of publication :
Journal of Virology
Authors :
Lavi, Uri
;
.
Volume :
14
Co-Authors:
Lavi, U., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Nattenberg, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Ronen, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Marcus, M., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Facilitators :
From page:
1337
To page:
1342
(
Total pages:
6
)
Abstract:
The virulent phage phie of B. subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III(ts) mutant cells infected with phi epsilon at restrictive temperatures do not support phage DNA synthesis. However, phi epsilon grows normally at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III+ revertants isolated from the mutant strain. Temperature shift down experiments with phi epsilon infected cells having thermosensitive DNA polymerase III (pol III(ts)) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature shift up experiments with phi epsilon infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that phi epsilon requires DNA polymerase III throughout its replication stage.
Note:
Related Files :
bacillus subtilis
DNA Nucleotidyltransferases
microorganism
mutation
temperature
virus DNA
virus replication
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30128
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
Scientific Publication
Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e
14
Lavi, U., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Nattenberg, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Ronen, A., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Marcus, M., Dept. Genet., Hebrew Univ., Jerusalem, Israel
Bacillus subtilis DNA polymerase III is required for the replication of the virulent bacteriophage phi e
The virulent phage phie of B. subtilis which contains hydroxymethyluracil in its DNA requires host DNA polymerase III for its DNA replication. DNA polymerase III(ts) mutant cells infected with phi epsilon at restrictive temperatures do not support phage DNA synthesis. However, phi epsilon grows normally at low and high temperatures in the mutant's parent strain and in spontaneous DNA polymerase III+ revertants isolated from the mutant strain. Temperature shift down experiments with phi epsilon infected cells having thermosensitive DNA polymerase III (pol III(ts)) indicate that at 48 C the thermolabile DNA polymerase III is irreversibly inactivated and has to be synthesized de novo after the shift to 37 C, before phage DNA synthesis can begin. Temperature shift up experiments with phi epsilon infected mutant cells show that phage replication is arrested immediately after the temperature shift and indicate that phi epsilon requires DNA polymerase III throughout its replication stage.
Scientific Publication
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