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Conditional repression of STAT5 expression during lactation reveals its exclusive roles in mammary gland morphology, milk-protein gene expression, and neonate growth
Year:
2011
Authors :
Barash, Itamar
;
.
Reichenstein, Moshe
;
.
Volume :
78
Co-Authors:
Reichenstein, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel
Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
585
To page:
596
(
Total pages:
12
)
Abstract:
The role of Stat5 in maintaining adequate lactation was studied in Stat5a -/- mice expressing a conditionally suppressed transgenic STAT5 in their mammary glands. This system enables distinguishing STAT5's effects on lactation from its contribution to mammary development during gestation. Females were allowed to express STAT5 during their first pregnancy. After delivery, STAT5 levels were manipulated by doxycycline administration and withdrawal. In two lines of genetically modified mice, the absence of STAT5 expression during the first 10 days of lactation resulted in a decrease of 29% or 41% in newborn weight gain. The STAT5-dependent decrease in growth was recoverable, but not completely reversible, particularly when STAT5 expression was omitted for the first 4 days of lactation. Within the first 10 days of STAT5-omitted lactation, alveolar occupancy regressed by 50% compared to that measured at delivery. By Day 10, only 18% of the fat-pad area was involved in milk production. The alveolar regression caused by 4 days of STAT5 deficiency was reversible, but neonate growth remained delayed. STAT5 deficiency resulted in reduced estrogen receptor α and connexin 32 gene expression, accompanied by delayed induction of both anti- and pro-apoptotic Bcl-2 family members. An increase in Gata-3 expression may reflect an attempt to maintain alveolar progenitors. A decrease of 39% and 23% in WAP and α-lactalbumin expression, respectively, with no associated effects on β-casein, also resulted from lack of STAT5 expression in the first 10 days of lactation. This deficiency enhances the major effect of alveolar regression on delayed weight gain in newborns. © 2011 Wiley-Liss, Inc.
Note:
Related Files :
animal experiment
Animals
Female
gene expression
Growth
lactation
mice
pregnancy
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More details
DOI :
10.1002/mrd.21345
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30137
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Conditional repression of STAT5 expression during lactation reveals its exclusive roles in mammary gland morphology, milk-protein gene expression, and neonate growth
78
Reichenstein, M., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel
Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel, Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel
Barash, I., Institute of Animal Science, ARO, The Volcani Center, Bet Dagan, Israel
Conditional repression of STAT5 expression during lactation reveals its exclusive roles in mammary gland morphology, milk-protein gene expression, and neonate growth
The role of Stat5 in maintaining adequate lactation was studied in Stat5a -/- mice expressing a conditionally suppressed transgenic STAT5 in their mammary glands. This system enables distinguishing STAT5's effects on lactation from its contribution to mammary development during gestation. Females were allowed to express STAT5 during their first pregnancy. After delivery, STAT5 levels were manipulated by doxycycline administration and withdrawal. In two lines of genetically modified mice, the absence of STAT5 expression during the first 10 days of lactation resulted in a decrease of 29% or 41% in newborn weight gain. The STAT5-dependent decrease in growth was recoverable, but not completely reversible, particularly when STAT5 expression was omitted for the first 4 days of lactation. Within the first 10 days of STAT5-omitted lactation, alveolar occupancy regressed by 50% compared to that measured at delivery. By Day 10, only 18% of the fat-pad area was involved in milk production. The alveolar regression caused by 4 days of STAT5 deficiency was reversible, but neonate growth remained delayed. STAT5 deficiency resulted in reduced estrogen receptor α and connexin 32 gene expression, accompanied by delayed induction of both anti- and pro-apoptotic Bcl-2 family members. An increase in Gata-3 expression may reflect an attempt to maintain alveolar progenitors. A decrease of 39% and 23% in WAP and α-lactalbumin expression, respectively, with no associated effects on β-casein, also resulted from lack of STAT5 expression in the first 10 days of lactation. This deficiency enhances the major effect of alveolar regression on delayed weight gain in newborns. © 2011 Wiley-Liss, Inc.
Scientific Publication
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