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An immunochemical analysis of oxytocin and vasopressin prohormone processing in vivo
Year:
1988
Source of publication :
peptides ( source)
Authors :
Altstein, Miriam
;
.
Volume :
9
Co-Authors:
Altstein, M., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Whitnall, M.H., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
House, S., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Key, S., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Gainer, H., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Facilitators :
From page:
87
To page:
105
(
Total pages:
19
)
Abstract:
Antisera against partially processed, unamidated forms of AVP and OT were raised and characterized by radioimmunoassay and immunocytochemistry. These antibodies, and antibodies that recognize fully processed, amidated forms of AVP and OT, were used together with various fractionation methods to study the content of prohormones, partially processed and fully processed forms of AVP and OT in the hypothalamo-neurohypophysial system of adult and fetal (E21) rats. The levels of cleaved AVP and OT in the fetus were lower than those of the adult (1 to 3 orders of magnitude for brain and pituitary, respectively), and the detection of cleaved OT in brain and pituitary was delayed compared to that of AVP. Pro-AVP cleavage efficiency in the adult and the fetus was high (99 and 95% cleavage, respectively) resulting in formation of fully processed amidated forms of AVP, with no detectable partially processed peptides. Pro-OT processing in the adult was very similar (over 99% cleavage) resulting in formation of fully processed amidated OT. However, Pro-OT processing efficiency in the fetus was very low and incomplete, resulting in 40% unprocessed precursor and the accumulation of C-terminally extended unamidated intermediate forms (OT-Gly, OT-Gly-Lys, and OT-Gly-Lys-Arg). © 1988.
Note:
Related Files :
adult
Animal
animal cell
Electrophoresis
fetus
histochemistry
Hypothalamo-Hypophyseal System
Male
Show More
Related Content
More details
DOI :
10.1016/0196-9781(88)90014-9
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30176
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
An immunochemical analysis of oxytocin and vasopressin prohormone processing in vivo
9
Altstein, M., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Whitnall, M.H., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
House, S., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Key, S., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
Gainer, H., Laboratory of Neurochemistry National Institute of Neurological and Communicative Disorders, Stroke National Institutes of Health, Bethesda, MD 20892, United States
An immunochemical analysis of oxytocin and vasopressin prohormone processing in vivo
Antisera against partially processed, unamidated forms of AVP and OT were raised and characterized by radioimmunoassay and immunocytochemistry. These antibodies, and antibodies that recognize fully processed, amidated forms of AVP and OT, were used together with various fractionation methods to study the content of prohormones, partially processed and fully processed forms of AVP and OT in the hypothalamo-neurohypophysial system of adult and fetal (E21) rats. The levels of cleaved AVP and OT in the fetus were lower than those of the adult (1 to 3 orders of magnitude for brain and pituitary, respectively), and the detection of cleaved OT in brain and pituitary was delayed compared to that of AVP. Pro-AVP cleavage efficiency in the adult and the fetus was high (99 and 95% cleavage, respectively) resulting in formation of fully processed amidated forms of AVP, with no detectable partially processed peptides. Pro-OT processing in the adult was very similar (over 99% cleavage) resulting in formation of fully processed amidated OT. However, Pro-OT processing efficiency in the fetus was very low and incomplete, resulting in 40% unprocessed precursor and the accumulation of C-terminally extended unamidated intermediate forms (OT-Gly, OT-Gly-Lys, and OT-Gly-Lys-Arg). © 1988.
Scientific Publication
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