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Mono‐ and bi‐phasic Salmonella typhi: genetic homogeneity and distinguishing characteristics
Year:
1992
Source of publication :
Molecular Microbiology
Authors :
Gafni, Yedidya
;
.
Rubinfeld, Ben Zion
;
.
Volume :
6
Co-Authors:
Moshitch, S., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Doll, L., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Rubinfeld, B.‐Z., Department of Plant Genetics and Breeding, Aro, Volcani Centre, PO Box 5, Bet Dagan, 50250, Israel
Stocker, B.A.D., Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305, United States, Howard Highes Medical Institute, Beckman Center, Stanford University School of Medicine, Stanford, California, 94305, United States
Schoolnik, G.K., Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305, United States, Howard Highes Medical Institute, Beckman Center, Stanford University School of Medicine, Stanford, California, 94305, United States
Gafni, Y., Department of Plant Genetics and Breeding, Aro, Volcani Centre, PO Box 5, Bet Dagan, 50250, Israel
Frankel, G., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
2589
To page:
2597
(
Total pages:
9
)
Abstract:
Several lines of evidence indicate a relatively low genetic heterogeneity in the natural Salmonella typhi population. However, some S. typhi isolates found in Indonesia express, instead of the usual fliC‐d flagellin gene, a different flagellar gene fliC‐j. In addition, Indonesian strains may have a second flagellar antigen fliC‐z66. We have previously suggested, on the basis of the flagellar antigen constitution, that S. typhi evolved in an isolated human population in Indonesia. In order to test this hypothesis, we have gathered S. typhi isolates from around the world and tested the genetic heterogeneity among them. In general, polymorphism was greater in isolates from the Far East, as was indicated by Southern hybridizations with rDNA and fliC DNA probes. Gene fliC‐j was not found in S. typhi isolates, other than those from Indonesia. However, the one‐clone origin of S. typhi was indicated by a common DNA fingerprint pattern and by the occurrence, in the 5′ end region of the fliC gene, of 10 scattered nucleotides that differ from the corresponding 10 nucleotides in other fliC alleles studied. These nucleotides were present in all isolates tested but did not change the amino acid sequence of the flagellin polypeptide. Copyright © 1992, Wiley Blackwell. All rights reserved
Note:
Related Files :
Base Sequence
Israel
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Show More
Related Content
More details
DOI :
10.1111/j.1365-2958.1992.tb01436.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30197
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
Scientific Publication
Mono‐ and bi‐phasic Salmonella typhi: genetic homogeneity and distinguishing characteristics
6
Moshitch, S., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Doll, L., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Rubinfeld, B.‐Z., Department of Plant Genetics and Breeding, Aro, Volcani Centre, PO Box 5, Bet Dagan, 50250, Israel
Stocker, B.A.D., Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305, United States, Howard Highes Medical Institute, Beckman Center, Stanford University School of Medicine, Stanford, California, 94305, United States
Schoolnik, G.K., Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305, United States, Howard Highes Medical Institute, Beckman Center, Stanford University School of Medicine, Stanford, California, 94305, United States
Gafni, Y., Department of Plant Genetics and Breeding, Aro, Volcani Centre, PO Box 5, Bet Dagan, 50250, Israel
Frankel, G., Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, 76100, Israel
Mono‐ and bi‐phasic Salmonella typhi: genetic homogeneity and distinguishing characteristics
Several lines of evidence indicate a relatively low genetic heterogeneity in the natural Salmonella typhi population. However, some S. typhi isolates found in Indonesia express, instead of the usual fliC‐d flagellin gene, a different flagellar gene fliC‐j. In addition, Indonesian strains may have a second flagellar antigen fliC‐z66. We have previously suggested, on the basis of the flagellar antigen constitution, that S. typhi evolved in an isolated human population in Indonesia. In order to test this hypothesis, we have gathered S. typhi isolates from around the world and tested the genetic heterogeneity among them. In general, polymorphism was greater in isolates from the Far East, as was indicated by Southern hybridizations with rDNA and fliC DNA probes. Gene fliC‐j was not found in S. typhi isolates, other than those from Indonesia. However, the one‐clone origin of S. typhi was indicated by a common DNA fingerprint pattern and by the occurrence, in the 5′ end region of the fliC gene, of 10 scattered nucleotides that differ from the corresponding 10 nucleotides in other fliC alleles studied. These nucleotides were present in all isolates tested but did not change the amino acid sequence of the flagellin polypeptide. Copyright © 1992, Wiley Blackwell. All rights reserved
Scientific Publication
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