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Regulation of oleoresinosis in grand fir (Abies grandis): Coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding
Year:
1994
Source of publication :
Plant physiology (source)
Authors :
Lewinsohn, Efraim
;
.
Volume :
106
Co-Authors:
Funk, C., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States, Botanical Institute, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland
Lewinsohn, E., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Vogel, B.S., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Steele, C.L., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Facilitators :
From page:
999
To page:
1005
(
Total pages:
7
)
Abstract:
Oleoresin (pitch) is a defensive secretion composed of monoterpene olefins (turpentine) and diterpene resin acids (rosin) that is produced in grand fir (Abies grandis Lindl.) stems in response to wounding. Monoterpene and diterpene biosynthesis are coordinately induced in wounded stems as determined by monitoring the activity of monoterpene and diterpene cyclases, as well as two cytochrome P450-dependent diterpenoid hydroxylases involved in the formation of (-)-abietic acid, the principal resin acid of this species. The activity of these enzymes reaches maximum levels that are 5- to 100-fold higher than those of nonwounded control stems 10 d after wounding and this is followed by a synchronous decline. The increase in biosynthetic activity is consequently followed by the accumulation of a viscous mass of resin acids, with the loss of the volatile monoterpenes, at the site of injury. The observed coordinate induction of monoterpene olefin and abietic acid biosynthesis and the results of oleoresin analysis are consistent with the role of the volatile monoterpenes as a solvent for the mobilization and deposition of resin acids at the wound site to seal the injury with a rosin barrier after the evaporation of the turpentine. The last step of resin acid biosynthesis is catalyzed by an operationally soluble aldehyde dehydrogenase that is not inducible by wounding but seemingly is expressed constitutively at a high level. In vivo [14C]acetate feeding and resin analysis indicate that this enzyme is not efficiently coupled to the earlier steps of the pathway.
Note:
Related Files :
Abies grandis
forest trees
grand fir
oleoresin
trees
Wounding
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30213
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:52
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Scientific Publication
Regulation of oleoresinosis in grand fir (Abies grandis): Coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding
106
Funk, C., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States, Botanical Institute, University of Basel, Hebelstrasse 1, 4056 Basel, Switzerland
Lewinsohn, E., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Vogel, B.S., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Steele, C.L., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Croteau, R., Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, United States
Regulation of oleoresinosis in grand fir (Abies grandis): Coordinate induction of monoterpene and diterpene cyclases and two cytochrome P450-dependent diterpenoid hydroxylases by stem wounding
Oleoresin (pitch) is a defensive secretion composed of monoterpene olefins (turpentine) and diterpene resin acids (rosin) that is produced in grand fir (Abies grandis Lindl.) stems in response to wounding. Monoterpene and diterpene biosynthesis are coordinately induced in wounded stems as determined by monitoring the activity of monoterpene and diterpene cyclases, as well as two cytochrome P450-dependent diterpenoid hydroxylases involved in the formation of (-)-abietic acid, the principal resin acid of this species. The activity of these enzymes reaches maximum levels that are 5- to 100-fold higher than those of nonwounded control stems 10 d after wounding and this is followed by a synchronous decline. The increase in biosynthetic activity is consequently followed by the accumulation of a viscous mass of resin acids, with the loss of the volatile monoterpenes, at the site of injury. The observed coordinate induction of monoterpene olefin and abietic acid biosynthesis and the results of oleoresin analysis are consistent with the role of the volatile monoterpenes as a solvent for the mobilization and deposition of resin acids at the wound site to seal the injury with a rosin barrier after the evaporation of the turpentine. The last step of resin acid biosynthesis is catalyzed by an operationally soluble aldehyde dehydrogenase that is not inducible by wounding but seemingly is expressed constitutively at a high level. In vivo [14C]acetate feeding and resin analysis indicate that this enzyme is not efficiently coupled to the earlier steps of the pathway.
Scientific Publication
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