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The tomato plastidic fructokinase SlFRK3 plays a role in xylem development
Year:
2016
Source of publication :
New Phytologist
Authors :
Damari-Weissler, Hila
;
.
German, Marcelo A.
;
.
Granot, David
;
.
Schaffer, Arthur
;
.
Stein, Ofer
;
.
Yeselson, Yelena
;
.
Volume :
209
Co-Authors:
Stein, O., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, The Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Damari-Weissler, H., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Secchi, F., Department of Plant Sciences, University of California, Davis, CA, United States
Rachamilevitch, S., Albert Katz Department of Dryland Biotechnologies, Blaustein Institutes for Desert Research, Ben Gurion University, Sede Boqer Campus, Israel
German, M.A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Yeselson, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Amir, R., Laboratory of Plant Science, Migal Galilee Research Center, PO Box 831, Kiryat Shmona, Israel
Schaffer, A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Holbrook, N.M., Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA, United States
Aloni, R., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel
Zwieniecki, M.A., Department of Plant Sciences, University of California, Davis, CA, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
1484
To page:
1495
(
Total pages:
12
)
Abstract:
Plants have two kinds of fructokinases (FRKs) that catalyze the key step of fructose phosphorylation, cytosolic and plastidic. The major cytosolic tomato FRK, SlFRK2, is essential for the development of xylem vessels. In order to study the role of SlFRK3, which encodes the only plastidic FRK, we generated transgenic tomato (Solanum lycopersicon) plants with RNAi suppression of SlFRK3 as well as plants expressing beta-glucoronidase (GUS) under the SlFRK3 promoter. GUS staining indicated SlFRK3 expression in vascular tissues of the leaves and stems, including cambium, differentiating xylem, young xylem fibers and phloem companion cells. Suppression of SlFRK3 reduced the stem xylem area, stem and root water conductance, and whole-plant transpiration, with minor effects on plant development. However, suppression of SlFRK3 accompanied by partial suppression of SlFRK2 induced significant growth-inhibition effects, including the wilting of mature leaves. Grafting experiments revealed that these growth effects are imposed primarily by the leaves, whose petioles had unlignified, thin-walled xylem fibers with collapsed parenchyma cells around the vessels. A cross between the SlFRK2-antisense and SlFRK3-RNAi lines exhibited similar wilting and anatomical effects, confirming that these effects are the result of the combined suppression of SlFRK3 and SlFRK2. These results demonstrate a role of the plastidic SlFRK3 in xylem development and hydraulic conductance. © 2016 New Phytologist Trust.
Note:
Related Files :
evapotranspiration
Genetics
metabolism
phenotype
RNA
transpiration
water
Show More
Related Content
More details
DOI :
10.1111/nph.13705
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30241
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
You may also be interested in
Scientific Publication
The tomato plastidic fructokinase SlFRK3 plays a role in xylem development
209
Stein, O., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel, The Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel
Damari-Weissler, H., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Secchi, F., Department of Plant Sciences, University of California, Davis, CA, United States
Rachamilevitch, S., Albert Katz Department of Dryland Biotechnologies, Blaustein Institutes for Desert Research, Ben Gurion University, Sede Boqer Campus, Israel
German, M.A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Yeselson, Y., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Amir, R., Laboratory of Plant Science, Migal Galilee Research Center, PO Box 831, Kiryat Shmona, Israel
Schaffer, A., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Holbrook, N.M., Department of Organismic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA, United States
Aloni, R., Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel
Zwieniecki, M.A., Department of Plant Sciences, University of California, Davis, CA, United States
Granot, D., Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
The tomato plastidic fructokinase SlFRK3 plays a role in xylem development
Plants have two kinds of fructokinases (FRKs) that catalyze the key step of fructose phosphorylation, cytosolic and plastidic. The major cytosolic tomato FRK, SlFRK2, is essential for the development of xylem vessels. In order to study the role of SlFRK3, which encodes the only plastidic FRK, we generated transgenic tomato (Solanum lycopersicon) plants with RNAi suppression of SlFRK3 as well as plants expressing beta-glucoronidase (GUS) under the SlFRK3 promoter. GUS staining indicated SlFRK3 expression in vascular tissues of the leaves and stems, including cambium, differentiating xylem, young xylem fibers and phloem companion cells. Suppression of SlFRK3 reduced the stem xylem area, stem and root water conductance, and whole-plant transpiration, with minor effects on plant development. However, suppression of SlFRK3 accompanied by partial suppression of SlFRK2 induced significant growth-inhibition effects, including the wilting of mature leaves. Grafting experiments revealed that these growth effects are imposed primarily by the leaves, whose petioles had unlignified, thin-walled xylem fibers with collapsed parenchyma cells around the vessels. A cross between the SlFRK2-antisense and SlFRK3-RNAi lines exhibited similar wilting and anatomical effects, confirming that these effects are the result of the combined suppression of SlFRK3 and SlFRK2. These results demonstrate a role of the plastidic SlFRK3 in xylem development and hydraulic conductance. © 2016 New Phytologist Trust.
Scientific Publication
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