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אסיף מאגר המחקר החקלאי
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A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota
Year:
2015
Source of publication :
PLoS genetics
Authors :
Chen, Lea
;
.
Evenor, Dalia
;
.
Fogel, Doron
;
.
Guenoune (Gelbart), Dana
;
.
Karniel, Uri
;
.
Kutsher, Yaarit
;
.
Lapidot, Moshe
;
.
Levin, Ilan
;
.
Machbash, Zion
;
.
Nahon, Saadia
;
.
Reuveni, Moshe
;
.
Shlomo, Haviva
;
.
Volume :
11
Co-Authors:
Lapidot, M., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Karniel, U., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gelbart, D., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fogel, D., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Evenor, D., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Kutsher, Y., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Makhbash, Z., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Nahon, S., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Shlomo, H., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Chen, L., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Reuveni, M., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Levin, I., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:
Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, the association between 27 polymorphic DNA markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with TYLCV in 51 segregating recombinant populations were analyzed. These analyses localized ty-5 into a 425 bp region containing two transversions: one in the first exon of a gene encoding the tomato homolog of the messenger RNA surveillance factor Pelota (Pelo), and a second in its proximal promoter. Analyses of susceptible and resistant lines revealed that the relative transcript level of the gene remained unchanged, regardless of whether the plants were infected with TYLCV or not. This suggests that the polymorphism discovered in the coding region of the gene controls the resistance. Silencing of Pelo in a susceptible line rendered the transgenic plants highly resistant, while in the resistant line TY172 had no effect on symptom development. In addition, over-expression of the susceptible allele of the gene in the resistant TY172 line rendered it susceptible, while over-expression of the resistant allele in susceptible plants had no effect. These results confirm that Pelo is the gene controlling resistance at the ty-5 locus. Pelo, implicated in the ribosome recycling-phase of protein synthesis, offers an alternative route to promote resistance to TYLCV and other viruses. © 2015 Lapidot et al.
Note:
Related Files :
chromosome mapping
disease resistance
gene expression
gene silencing
genetic markers
Genetics
molecular genetics
Tomato yellow leaf curl virus
Show More
Related Content
More details
DOI :
10.1371/journal.pgen.1005538
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30258
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
You may also be interested in
Scientific Publication
A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota
11
Lapidot, M., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Karniel, U., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Gelbart, D., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Fogel, D., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Evenor, D., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Kutsher, Y., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Makhbash, Z., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Nahon, S., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Shlomo, H., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Chen, L., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Reuveni, M., Department of Ornamental Plants and Agricultural Biotechnology, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Levin, I., Department of Vegetable and Field Crop Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
A Novel Route Controlling Begomovirus Resistance by the Messenger RNA Surveillance Factor Pelota
Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. The TYLCV-resistant line TY172 carries a major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, the association between 27 polymorphic DNA markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with TYLCV in 51 segregating recombinant populations were analyzed. These analyses localized ty-5 into a 425 bp region containing two transversions: one in the first exon of a gene encoding the tomato homolog of the messenger RNA surveillance factor Pelota (Pelo), and a second in its proximal promoter. Analyses of susceptible and resistant lines revealed that the relative transcript level of the gene remained unchanged, regardless of whether the plants were infected with TYLCV or not. This suggests that the polymorphism discovered in the coding region of the gene controls the resistance. Silencing of Pelo in a susceptible line rendered the transgenic plants highly resistant, while in the resistant line TY172 had no effect on symptom development. In addition, over-expression of the susceptible allele of the gene in the resistant TY172 line rendered it susceptible, while over-expression of the resistant allele in susceptible plants had no effect. These results confirm that Pelo is the gene controlling resistance at the ty-5 locus. Pelo, implicated in the ribosome recycling-phase of protein synthesis, offers an alternative route to promote resistance to TYLCV and other viruses. © 2015 Lapidot et al.
Scientific Publication
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