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Phenotypic and genetic diversity among rhizobia isolated from three Hedysarum species: H. spinosissimum, H. coronarium and H. flexuosum
Year:
2003
Source of publication :
Plant and Soil
Authors :
Nemas, Haya
;
.
Volume :
251
Co-Authors:
Kishinevsky, B.D., Dept. of Agronomy/Natural Resources, ARO, Volcani Center, Bet Dagan 50-250, Israel
Nandasena, K.G., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia
Yates, R.J., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia
Nemas, C., Dept. of Agronomy/Natural Resources, ARO, Volcani Center, Bet Dagan 50-250, Israel
Howieson, J.G., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia, W. Australian Dept. of Agriculture, South Perth, WA 6151, Australia
Facilitators :
From page:
143
To page:
153
(
Total pages:
11
)
Abstract:
Cultural, physiological and biochemical properties of 18 strains of rhizobia isolated from root nodules of the forage legume H. spinosissimum were compared with those of rhizobia from the related species H. coronarium (15 strains) and H. flexuosum (four strains). On the basis of 43 characteristics the 37 strains of Hedysarum rhizobia could be divided into two groups by numerical analysis. The H. spinosissimum rhizobia formed the first group and the second group comprised the strains from H. coronarium and H. flexuosum. The reference Rhizobium leguminosarum bv. viceae strain 250A was clustered with the rhizobia from H. coronarium and H. flexuosum. By contrast Bradyrhizobium sp. (Arachis) reference strain 280A was not clustered with any of the strains tested, indicating that the H. spinosissimum rhizobia differ from both Rhizobium and Bradyrhizobium. Serological data also discriminate between H. spinosissimum and H. coronarium rhizobia but not between the latter and H. flexuosum strains. The strains tested exhibit a high degree of specificity for nodulation and nitrogen fixation. We also determined the16SrRNA gene sequence of H. spinosissimum rhizobia (four strains), H. coronarium (two strains) and H. flexuosum (two strains) and found that the four H. spinosissimum isolates share a 98% identity among each other in this region but they showed less than 92% identity to the H. coronarium and H. flexuosum isolates. The H. spinosissimum isolates were closely related to both Mesorhizobium loti and M. ciceri, sharing 97% identity with each species.
Note:
Related Files :
Arachis
Bradyrhizobium sp.
Hedysarum spp.
Mesorhizobium loti
phenotype
Prokaryota
Rhizobium
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Related Content
More details
DOI :
10.1023/A:1022967213088
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30264
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
Scientific Publication
Phenotypic and genetic diversity among rhizobia isolated from three Hedysarum species: H. spinosissimum, H. coronarium and H. flexuosum
251
Kishinevsky, B.D., Dept. of Agronomy/Natural Resources, ARO, Volcani Center, Bet Dagan 50-250, Israel
Nandasena, K.G., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia
Yates, R.J., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia
Nemas, C., Dept. of Agronomy/Natural Resources, ARO, Volcani Center, Bet Dagan 50-250, Israel
Howieson, J.G., Centre for Rhizobium Studies, Murdoch University, South Perth, WA 6151, Australia, W. Australian Dept. of Agriculture, South Perth, WA 6151, Australia
Phenotypic and genetic diversity among rhizobia isolated from three Hedysarum species: H. spinosissimum, H. coronarium and H. flexuosum
Cultural, physiological and biochemical properties of 18 strains of rhizobia isolated from root nodules of the forage legume H. spinosissimum were compared with those of rhizobia from the related species H. coronarium (15 strains) and H. flexuosum (four strains). On the basis of 43 characteristics the 37 strains of Hedysarum rhizobia could be divided into two groups by numerical analysis. The H. spinosissimum rhizobia formed the first group and the second group comprised the strains from H. coronarium and H. flexuosum. The reference Rhizobium leguminosarum bv. viceae strain 250A was clustered with the rhizobia from H. coronarium and H. flexuosum. By contrast Bradyrhizobium sp. (Arachis) reference strain 280A was not clustered with any of the strains tested, indicating that the H. spinosissimum rhizobia differ from both Rhizobium and Bradyrhizobium. Serological data also discriminate between H. spinosissimum and H. coronarium rhizobia but not between the latter and H. flexuosum strains. The strains tested exhibit a high degree of specificity for nodulation and nitrogen fixation. We also determined the16SrRNA gene sequence of H. spinosissimum rhizobia (four strains), H. coronarium (two strains) and H. flexuosum (two strains) and found that the four H. spinosissimum isolates share a 98% identity among each other in this region but they showed less than 92% identity to the H. coronarium and H. flexuosum isolates. The H. spinosissimum isolates were closely related to both Mesorhizobium loti and M. ciceri, sharing 97% identity with each species.
Scientific Publication
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