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A new dietary model to study colorectal carcinogenesis: Experimental design, food preparation, and experimental findings
Year:
1996
Source of publication :
Nutrition and Cancer
Authors :
Angel, Samuel
;
.
Volume :
25
Co-Authors:
Rozen, P., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Liberman, V., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Lubin, F., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Angel, S., Department of Food Science, Agricultural Research Organization, Ministry of Agriculture, Bet Degan, Israel
Owen, R., Div. Toxicol. Cancer Risk Factors, German Cancer Research Center, Heidelberg D-69120, Germany
Trostler, N., Dept. Biochem., Food Sci., and Nutr., Hebrew University, Rehovot, Israel
Shkolnik, T., Clinical and Research Laboratories, Nahariya Hospital, Nahariya, 22100, Israel
Kritchevsky, D., Wistar Institute, Philadelphia, PA 19104, United States
Facilitators :
From page:
79
To page:
100
(
Total pages:
22
)
Abstract:
Experimental dietary studies of human colorectal carcinogenesis are usually based on the AIN-76A diet, which is dissimilar to human food in source, preparation, and content. The aims of this study were to examine the feasibility of preparing and feeding rats the diet of a specific human population at risk for colorectal neoplasia and to determine whether changes in the colonic morphology and metabolic contents would differ from those resulting from a standard rat diet. The mean daily food intake composition of a previously evaluated adenoma patient case-control study was used for the 'human adenoma' (HA) experimental diet. Foods were prepared as for usual human consumption and processed by dehydration to the physical characteristics of an animal diet. Sixty-four female Sprague-Dawley rats were randomized and fed ad libitum the HA or the AIN-76A diet. Every eight weeks, eight rats from each group were sacrificed, and the colons and contents were examined. Analysis of the prepared food showed no significant deleterious changes; food intake and weight gain were similar in both groups. Compared with the controls, the colonic contents of rats fed the HA diet contained significantly less calcium, concentrations of neutral sterols, total lipids, and cholic and deoxycholic acids were increased, and there were no colonic histological changes other than significant epithelial hyperproliferation. This initial study demonstrated that the HA diet can be successfully processed for feeding to experimental animals and is acceptable and adequate for growth but induces significant metabolic and hyperproliferative changes in the rat colon. This dietary model may be useful for studies of human food, narrowing the gap between animal experimentation and human nutritional research.
Note:
Related Files :
animal experiment
animal model
Animals
Carcinogenesis
Cell Proliferation
fatty acids
Female
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30266
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
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Scientific Publication
A new dietary model to study colorectal carcinogenesis: Experimental design, food preparation, and experimental findings
25
Rozen, P., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Liberman, V., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Lubin, F., Gastroenterology Department, Tel Aviv Medical Center, Tel Aviv University, Tel Aviv 64239, Israel
Angel, S., Department of Food Science, Agricultural Research Organization, Ministry of Agriculture, Bet Degan, Israel
Owen, R., Div. Toxicol. Cancer Risk Factors, German Cancer Research Center, Heidelberg D-69120, Germany
Trostler, N., Dept. Biochem., Food Sci., and Nutr., Hebrew University, Rehovot, Israel
Shkolnik, T., Clinical and Research Laboratories, Nahariya Hospital, Nahariya, 22100, Israel
Kritchevsky, D., Wistar Institute, Philadelphia, PA 19104, United States
A new dietary model to study colorectal carcinogenesis: Experimental design, food preparation, and experimental findings
Experimental dietary studies of human colorectal carcinogenesis are usually based on the AIN-76A diet, which is dissimilar to human food in source, preparation, and content. The aims of this study were to examine the feasibility of preparing and feeding rats the diet of a specific human population at risk for colorectal neoplasia and to determine whether changes in the colonic morphology and metabolic contents would differ from those resulting from a standard rat diet. The mean daily food intake composition of a previously evaluated adenoma patient case-control study was used for the 'human adenoma' (HA) experimental diet. Foods were prepared as for usual human consumption and processed by dehydration to the physical characteristics of an animal diet. Sixty-four female Sprague-Dawley rats were randomized and fed ad libitum the HA or the AIN-76A diet. Every eight weeks, eight rats from each group were sacrificed, and the colons and contents were examined. Analysis of the prepared food showed no significant deleterious changes; food intake and weight gain were similar in both groups. Compared with the controls, the colonic contents of rats fed the HA diet contained significantly less calcium, concentrations of neutral sterols, total lipids, and cholic and deoxycholic acids were increased, and there were no colonic histological changes other than significant epithelial hyperproliferation. This initial study demonstrated that the HA diet can be successfully processed for feeding to experimental animals and is acceptable and adequate for growth but induces significant metabolic and hyperproliferative changes in the rat colon. This dietary model may be useful for studies of human food, narrowing the gap between animal experimentation and human nutritional research.
Scientific Publication
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