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Isolation of a novel collagen gene (Mj-col-5) in Meloidogyne javanica and analysis of its expression pattern
Year:
2001
Source of publication :
Journal of Parasitology
Authors :
Chejanovsky, Nor
;
.
Koltai, Hinanit
;
.
Liu, Jia
;
.
Spiegel, Yitzhak
;
.
Volume :
87
Co-Authors:
Liu, J., Department of Entomology, Agriculture Research Organization, Volcani Center, Bet Dagan 50-250, Israel
Koltai, H., Department of Entomology, Agriculture Research Organization, Volcani Center, Bet Dagan 50-250, Israel
Chejanovsky, N., Department of Entomology, Agriculture Research Organization, Volcani Center, Bet Dagan 50-250, Israel
Spiegel, Y., Department of Entomology, Agriculture Research Organization, Volcani Center, Bet Dagan 50-250, Israel
Facilitators :
From page:
801
To page:
807
(
Total pages:
7
)
Abstract:
Mj-col-5, isolated from the plant parasitic nematode Meloidogyne javanica, has a longer carboxy-terminus than other members of the Caenorhabditis elegans COL-6 subfamily of cuticle collagen, including an extra tyrosine residue, and may form altered nonreducible cross-linkages. By semiquantitative determination at different life stages, Mj-col-5 transcript was shown to be more abundant in eggs than in juveniles/young females and adult females. To characterize further this gene's contribution to the changing cuticle of the nematode, we expressed a fusion protein containing a nonconserved 58-amino-acid sequence from the putative Mj-col-5 gene product and raised rabbit antiserum against the fusion protein. The antiserum detected a strongly reacting band (36 kDa, designated MJE36) on western blots of M. javanica eggs extracted with β-mercaptoethanol. MJE36 was sensitive to collagenase and was not detected on western blots of extracts from M. javanica second-stage juveniles or adult females. A band of the same molecular size was detected in Meloidogyne incognita egg extracts but not in those of Heterodera avenae. Immunoblot indicated that MJE36 is not present in egg shells of M. javanica.
Note:

The correct link to the article:

https://bioone.org/journals/Journal-of-Parasitology/volume-87/issue-4/0022-3395(2001)087[0801:IOANCG]2.0.CO;2/Isolation-of-a-Novel-Collagen-Gene-iMJ-COL-5-i/10.1645/0022-3395(2001)087[0801:IOANCG]2.0.CO;2.short

Related Files :
Animals
carboxy terminal sequence
Female
gene expression
genetic analysis
Parasite
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More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30279
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
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