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Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase
Year:
2011
Source of publication :
Chemosphere
Authors :
Kumari, Puja
;
.
Volume :
83
Co-Authors:
Trivedi, N., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Gupta, V., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Kumar, M., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Kumari, P., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Jha, B., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Facilitators :
From page:
706
To page:
712
(
Total pages:
7
)
Abstract:
The organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. Of the 19 marine bacterial isolates examined for their solvent tolerance at 10vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. The phylogenetic analysis of this strain using 16S rDNA sequence revealed 99% homology with Bacillus aquimaris. The cellulase enzyme secreted by this strain under normal conditions showed an optimum activity at pH 11 and 45°C. The enzyme did show functional stability even at higher pH (12) and temperature (75°C) with residual activity of 85% and 95% respectively. The enzyme activity in the presence of different additives were in the following order: Co+2>Fe+2>NaOCl2>CuSO4>KCl>NaCl. The enzyme stability in the presence of solvents at 20vol.% concentration was highest in benzene with 122% followed by methanol (85%), acetone (75%), toluene (73%) and heptane (42%). The pre-incubation of enzyme in ionic liquids such as 1-ethyl-3-methylimidazolium methanesulfonate and 1-ethyl-3-methylimidazolium bromide increased its activity to 150% and 155% respectively. The change in fatty acid profile with different solvents further elucidated the physiological adaptations of the strain to tolerate such extreme conditions. © 2011 Elsevier Ltd.
Note:
Related Files :
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More details
DOI :
10.1016/j.chemosphere.2011.02.006
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30322
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
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Scientific Publication
Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase
83
Trivedi, N., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Gupta, V., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Kumar, M., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Kumari, P., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Reddy, C.R.K., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Jha, B., Discipline of Marine Biotechnology and Ecology, Central Salt and Marine Chemicals Research Institute, Council of Scientific and Industrial Research (CSIR), Bhavnagar 364 021, India
Solvent tolerant marine bacterium Bacillus aquimaris secreting organic solvent stable alkaline cellulase
The organic solvent tolerant bacteria with their physiological abilities to decontaminate the organic pollutants have potentials to secrete extracellular enzymes of commercial importance. Of the 19 marine bacterial isolates examined for their solvent tolerance at 10vol.% concentration, one had the significant tolerance and showed a relative growth yield of 86% for acetone, 71% for methanol, 52% for benzene, 35% for heptane, 24% for toluene and 19% for ethylacetate. The phylogenetic analysis of this strain using 16S rDNA sequence revealed 99% homology with Bacillus aquimaris. The cellulase enzyme secreted by this strain under normal conditions showed an optimum activity at pH 11 and 45°C. The enzyme did show functional stability even at higher pH (12) and temperature (75°C) with residual activity of 85% and 95% respectively. The enzyme activity in the presence of different additives were in the following order: Co+2>Fe+2>NaOCl2>CuSO4>KCl>NaCl. The enzyme stability in the presence of solvents at 20vol.% concentration was highest in benzene with 122% followed by methanol (85%), acetone (75%), toluene (73%) and heptane (42%). The pre-incubation of enzyme in ionic liquids such as 1-ethyl-3-methylimidazolium methanesulfonate and 1-ethyl-3-methylimidazolium bromide increased its activity to 150% and 155% respectively. The change in fatty acid profile with different solvents further elucidated the physiological adaptations of the strain to tolerate such extreme conditions. © 2011 Elsevier Ltd.
Scientific Publication
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