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Transcriptomic Profiling of Apple in Response to Inoculation with a Pathogen (Penicillium expansum) and a Non-pathogen (Penicillium digitatum)
Year:
2014
Source of publication :
Plant Molecular Biology Reporter
Authors :
Droby, Samir
;
.
Volume :
32
Co-Authors:
Vilanova, L., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Wisniewski, M., Appalachian Fruit Research Station, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Wiltshire Road 2217, 25430 Kearneysville, WV, United States
Norelli, J., Appalachian Fruit Research Station, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Wiltshire Road 2217, 25430 Kearneysville, WV, United States
Viñas, I., Food Technology Department, Lleida University, XaRTA-Postharvest, Agrotecnio Center, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Torres, R., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Usall, J., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Phillips, J., Eastern Reional Research Center, US Department of Agriculture-Agricultural Research Service (USDA-ARS), 600 E Mermaid LN 2217, 25430 Wyndmoor, PA, United States
Droby, S., Department of Postharvest Science, ARO, the Volcani Center, PO Box 6, 50250 Bet Dagan, Israel
Teixidó, N., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Facilitators :
From page:
566
To page:
583
(
Total pages:
18
)
Abstract:
Penicillium expansum, the causal agent of blue mould of pome fruits, is a major postharvest pathogen in all producing countries. To develop a better understanding of disease resistance mechanisms in apples, a comprehensive transcriptional analysis of apple gene expression in response to a compatible (P. expansum) and non-host (Penicillium digitatum) pathogen was conducted using an apple microarray of approximately 40,000 probes. The resulting data provide further evidence that apples inoculated with P. expansum exhibit significant upregulation of defense-related genes and genes involved in detoxification of reactive oxygen species. In contrast, apples inoculated with P. digitatum, a non-host pathogen, exhibited upregulation of genes involved in phenylpropanoid metabolism. To confirm the accuracy of the expression profiles obtained with the microarray, reverse transcriptase-quantitative polymerase chain reaction was conducted for four genes specifically in the phenylpropanoid pathway. Expression data was obtained for different time points and fruit maturity stages. The highest expression level of the phenylpropanoid genes was detected 48 h after inoculation with P. expansum in both immature and mature apples. These results support the hypothesis that apples respond in a complex and diverse manner to the compatible compared to the non-host pathogen. To the best of our knowledge, this is the first study in apple fruit that has conducted an analysis of global changes in gene expression in response to a compatible (P. expansum) and non-host (P. digitatum) pathogen. © 2013 Springer Science+Business Media New York.
Note:
Related Files :
Blue mold
gene expression
green mold
Maturity stage
Microarray analysis
Phenylpropanoid pathway
Show More
Related Content
More details
DOI :
10.1007/s11105-013-0676-y
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30323
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
You may also be interested in
Scientific Publication
Transcriptomic Profiling of Apple in Response to Inoculation with a Pathogen (Penicillium expansum) and a Non-pathogen (Penicillium digitatum)
32
Vilanova, L., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Wisniewski, M., Appalachian Fruit Research Station, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Wiltshire Road 2217, 25430 Kearneysville, WV, United States
Norelli, J., Appalachian Fruit Research Station, US Department of Agriculture-Agricultural Research Service (USDA-ARS), Wiltshire Road 2217, 25430 Kearneysville, WV, United States
Viñas, I., Food Technology Department, Lleida University, XaRTA-Postharvest, Agrotecnio Center, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Torres, R., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Usall, J., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Phillips, J., Eastern Reional Research Center, US Department of Agriculture-Agricultural Research Service (USDA-ARS), 600 E Mermaid LN 2217, 25430 Wyndmoor, PA, United States
Droby, S., Department of Postharvest Science, ARO, the Volcani Center, PO Box 6, 50250 Bet Dagan, Israel
Teixidó, N., XaRTA-Postharvest, IRTA, Rovira Roure 191, 25198 Lleida, Catalonia, Spain
Transcriptomic Profiling of Apple in Response to Inoculation with a Pathogen (Penicillium expansum) and a Non-pathogen (Penicillium digitatum)
Penicillium expansum, the causal agent of blue mould of pome fruits, is a major postharvest pathogen in all producing countries. To develop a better understanding of disease resistance mechanisms in apples, a comprehensive transcriptional analysis of apple gene expression in response to a compatible (P. expansum) and non-host (Penicillium digitatum) pathogen was conducted using an apple microarray of approximately 40,000 probes. The resulting data provide further evidence that apples inoculated with P. expansum exhibit significant upregulation of defense-related genes and genes involved in detoxification of reactive oxygen species. In contrast, apples inoculated with P. digitatum, a non-host pathogen, exhibited upregulation of genes involved in phenylpropanoid metabolism. To confirm the accuracy of the expression profiles obtained with the microarray, reverse transcriptase-quantitative polymerase chain reaction was conducted for four genes specifically in the phenylpropanoid pathway. Expression data was obtained for different time points and fruit maturity stages. The highest expression level of the phenylpropanoid genes was detected 48 h after inoculation with P. expansum in both immature and mature apples. These results support the hypothesis that apples respond in a complex and diverse manner to the compatible compared to the non-host pathogen. To the best of our knowledge, this is the first study in apple fruit that has conducted an analysis of global changes in gene expression in response to a compatible (P. expansum) and non-host (P. digitatum) pathogen. © 2013 Springer Science+Business Media New York.
Scientific Publication
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