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Establishment of an agrobacterium-mediated transformation system for grape (Vitts vinifera l.): The role of antioxidants during grzpe-agrobacterium interactions
Year:
1996
Source of publication :
Nature Biotechnology
Authors :
Abu-Abied, Mohamad
;
.
Holland, Doron
;
.
Volume :
14
Co-Authors:
Perl, A., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Lotan, O., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Abu-Abied, M., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Facilitators :
From page:
624
To page:
628
(
Total pages:
5
)
Abstract:
Very short exposures of embryogenic calli of Vitis vinifera cv. Superior Seedless grape plants to diluted cultures of Agrobacterium resulted in plant tissue necrosis and subsequent cell death. Antibiotics used for Agrobacterium elimination or as plant selectable markers were not responsible for this necrotic response. Rather, cell death seemed to be oxygen-dependent and correlated with elevated levels of peroxides. Therefore, we studied the effects on necrosis of various combinations of antioxidants during and after grape-Agrobacterium cocultivation. The combination of polyvinylpolypyrrolidone and dithiothreitol was found to improve plant viability. Tissue necrosis was completely inhibited by these antioxidants while Agrobacterium virulence was not effected. These treatments enabled the recovery of stable transgenic grape plants resistant to hygromycin. © 1996 Nature Publishing Group.
Note:
Related Files :
Agriculture
Agrobacterium
antioxidants
bacterium culture
biotechnology
dithiothreitol
genetic transformation
grape
Show More
Related Content
More details
DOI :
10.1038/nbt0596-624
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30367
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:54
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Scientific Publication
Establishment of an agrobacterium-mediated transformation system for grape (Vitts vinifera l.): The role of antioxidants during grzpe-agrobacterium interactions
14
Perl, A., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Lotan, O., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Abu-Abied, M., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Holland, D., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, P. O. Box 6, Bet-Dagan, 50250, Israel
Establishment of an agrobacterium-mediated transformation system for grape (Vitts vinifera l.): The role of antioxidants during grzpe-agrobacterium interactions
Very short exposures of embryogenic calli of Vitis vinifera cv. Superior Seedless grape plants to diluted cultures of Agrobacterium resulted in plant tissue necrosis and subsequent cell death. Antibiotics used for Agrobacterium elimination or as plant selectable markers were not responsible for this necrotic response. Rather, cell death seemed to be oxygen-dependent and correlated with elevated levels of peroxides. Therefore, we studied the effects on necrosis of various combinations of antioxidants during and after grape-Agrobacterium cocultivation. The combination of polyvinylpolypyrrolidone and dithiothreitol was found to improve plant viability. Tissue necrosis was completely inhibited by these antioxidants while Agrobacterium virulence was not effected. These treatments enabled the recovery of stable transgenic grape plants resistant to hygromycin. © 1996 Nature Publishing Group.
Scientific Publication
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