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Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization
Year:
2001
Source of publication :
Physiologia Plantarum
Authors :
Holland, Doron
;
.
Volume :
113
Co-Authors:
Yan Zhong, G., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sisler, E.C., Department of Biochemistry, North Carolina State University, Raleigh, NC 17695-7622, United States
Holland, D., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Facilitators :
From page:
267
To page:
274
(
Total pages:
8
)
Abstract:
A simple new method, competitive hybridization, for identification of differentially regulated genes was used to isolate novel genes induced by ethylene in citrus (Citrus sinensis [L.] Osbeck cv. Shamouti) leaves. One of the isolated genes, an ethylene-induced esterase gene (EIE), was further characterized. The deduced protein sequence of this gene shows a similarity to those of several plant α/β hydrolase gene family members, which are known to be involved in secondary metabolism. Northern blot analysis demonstrated that EIE mRNA was induced by ethylene within 4 h and accumulated to a very high level 24 h after the initiation of ethylene treatment. Induction of EIE by ethylene could be counteracted by 1-methylcyclopropene, a potent ethylene perception inhibitor, indicating that the expression of EIE is ethylene-dependent. The bacterially expressed protein of EIE was recognized by antiserum against Pir7b, a naphthol AS esterase induced in rice by the non-host pathogen, Pseudomonas syringae pv. syringae. The EIE protein was identified in ethylene-treated leaves using anti-Pir7b anti-bodies. An α-naphthyl acetate esterase accumulated concomitantly with the increase in EIE protein in ethylene-treated citrus leaves. An enzyme activity assay followed by western analysis confirmed that the esterase was EIE.
Note:
Related Files :
1 methylcyclopropene
Bioassay
enzymes
Genes
hybridization
metabolism
plant
RNA
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Related Content
More details
DOI :
10.1034/j.1399-3054.2001.1130215.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30392
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:54
You may also be interested in
Scientific Publication
Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization
113
Yan Zhong, G., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Goren, R., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Riov, J., Kennedy-Leigh Centre for Horticultural Research, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, Rehovot 76100, Israel
Sisler, E.C., Department of Biochemistry, North Carolina State University, Raleigh, NC 17695-7622, United States
Holland, D., Agricultural Research Organization, Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Characterization of an ethylene-induced esterase gene isolated from Citrus sinensis by competitive hybridization
A simple new method, competitive hybridization, for identification of differentially regulated genes was used to isolate novel genes induced by ethylene in citrus (Citrus sinensis [L.] Osbeck cv. Shamouti) leaves. One of the isolated genes, an ethylene-induced esterase gene (EIE), was further characterized. The deduced protein sequence of this gene shows a similarity to those of several plant α/β hydrolase gene family members, which are known to be involved in secondary metabolism. Northern blot analysis demonstrated that EIE mRNA was induced by ethylene within 4 h and accumulated to a very high level 24 h after the initiation of ethylene treatment. Induction of EIE by ethylene could be counteracted by 1-methylcyclopropene, a potent ethylene perception inhibitor, indicating that the expression of EIE is ethylene-dependent. The bacterially expressed protein of EIE was recognized by antiserum against Pir7b, a naphthol AS esterase induced in rice by the non-host pathogen, Pseudomonas syringae pv. syringae. The EIE protein was identified in ethylene-treated leaves using anti-Pir7b anti-bodies. An α-naphthyl acetate esterase accumulated concomitantly with the increase in EIE protein in ethylene-treated citrus leaves. An enzyme activity assay followed by western analysis confirmed that the esterase was EIE.
Scientific Publication
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