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Demethylation of CpG islands in embryonic cells
Year:
1991
Source of publication :
Nature
Authors :
Shani, Moshe
;
.
Volume :
351
Co-Authors:
Frank, D., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Keshet, I., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Shani, M., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel, Institute of Animal Sciences, Volcani Center, Bet-Dagon, Israel
Levine, A., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Razin, A., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Cedar, H., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Facilitators :
From page:
239
To page:
241
(
Total pages:
3
)
Abstract:
DNA in differentiated somatic cells has a fixed pattern of methylation, which is faithfully copied after replication. By contrast, the methylation patterns of many tissue-specific and some housekeeping genes are altered during normal development1. This modification of DNA methylation in the embryo has also been observed in transgenic mice and in transfection experiments2. Here we report the fate in mice of an in vitro-methylated adenine phosphoribosyltransferase transgene. The entire 5′ CpG island region became demethylated, whereas the 3′ end of the gene remained modified and was even methylated de novo at additional sites. Transfection experiments in vitro show that the demethylation is rapid, is specific for embryonic cell-types and affects a variety of different CpG island sequences. This suggests that gene sequences can be recognized in the early embryo and imprinted with the correct methylation pattern through a combination of demethylation and de novo methylation. © 1991 Nature Publishing Group.
Note:
Related Files :
Animal
animal cell
Cricetinae
demethylation
dna methylation
embryo
gene expression regulation
mice
mouse
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30405
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:54
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Scientific Publication
Demethylation of CpG islands in embryonic cells
351
Frank, D., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Keshet, I., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Shani, M., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel, Institute of Animal Sciences, Volcani Center, Bet-Dagon, Israel
Levine, A., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Razin, A., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Cedar, H., Department of Cellular Biochemistry, Hewbrew University Medical School, Post Office Box 1172, Jerusalem, Israel
Demethylation of CpG islands in embryonic cells
DNA in differentiated somatic cells has a fixed pattern of methylation, which is faithfully copied after replication. By contrast, the methylation patterns of many tissue-specific and some housekeeping genes are altered during normal development1. This modification of DNA methylation in the embryo has also been observed in transgenic mice and in transfection experiments2. Here we report the fate in mice of an in vitro-methylated adenine phosphoribosyltransferase transgene. The entire 5′ CpG island region became demethylated, whereas the 3′ end of the gene remained modified and was even methylated de novo at additional sites. Transfection experiments in vitro show that the demethylation is rapid, is specific for embryonic cell-types and affects a variety of different CpG island sequences. This suggests that gene sequences can be recognized in the early embryo and imprinted with the correct methylation pattern through a combination of demethylation and de novo methylation. © 1991 Nature Publishing Group.
Scientific Publication
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