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Further purification and characterization of threadlike particles associated with the Citrus Tristeza disease
Year:
1972
Source of publication :
Virology
Authors :
Bar-Joseph, Moshe
;
.
Cohen, Jacob
;
.
Loebenstein, Gad
;
.
Volume :
50
Co-Authors:
Bar-Joseph, M., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Loebenstein, G., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Cohen, J., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
821
To page:
828
(
Total pages:
8
)
Abstract:
Additional purification of threadlike particles (TLP) from leaves and bark of Egyptian sour lime, Citrus aurantifolia (Christm.) Swing., and bark of Palestinian sweet lime, C. limmetioides Tanaka, infected with Citrus Tristeza disease, was achieved by density gradient centrifugation in cesium chloride, after fixation with formaldehyde. A buoyant density of 1.328 g/cm3 was determined by the use of two viral markers. The UV adsorption spectrum of TLP was typical of a nucleoprotein, and the 260:280 ratio of 1.21 indicated a nucleic acid content of ca 6%. TLP contained RNA but no DNA and a single protein species with a molecular weight of 25,000 ± 1000. TLP are helically constructed with a basic pitch of 37 + 2 Å and ten subunits in each turn of the basic helix. A molecular weight of 144 ± 23 × 106 for a particle with a normal length of 2000 nm was estimated. These properties of TLP, resembling those of other filamentous viruses, as well as their presence in infected tissues only, lend strength to the suggestion that TLP are the causal agent of the tristeza disease. © 1972.
Note:
Related Files :
Centrifugation, Density Gradient
Cesium
densitometry
Nucleoproteins
Plant Disease
Plant Diseases
Staining and Labeling
virus RNA
Show More
Related Content
More details
DOI :
10.1016/0042-6822(72)90436-9
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30487
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
Scientific Publication
Further purification and characterization of threadlike particles associated with the Citrus Tristeza disease
50
Bar-Joseph, M., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Loebenstein, G., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Cohen, J., Virus Laboratory, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel
Further purification and characterization of threadlike particles associated with the Citrus Tristeza disease
Additional purification of threadlike particles (TLP) from leaves and bark of Egyptian sour lime, Citrus aurantifolia (Christm.) Swing., and bark of Palestinian sweet lime, C. limmetioides Tanaka, infected with Citrus Tristeza disease, was achieved by density gradient centrifugation in cesium chloride, after fixation with formaldehyde. A buoyant density of 1.328 g/cm3 was determined by the use of two viral markers. The UV adsorption spectrum of TLP was typical of a nucleoprotein, and the 260:280 ratio of 1.21 indicated a nucleic acid content of ca 6%. TLP contained RNA but no DNA and a single protein species with a molecular weight of 25,000 ± 1000. TLP are helically constructed with a basic pitch of 37 + 2 Å and ten subunits in each turn of the basic helix. A molecular weight of 144 ± 23 × 106 for a particle with a normal length of 2000 nm was estimated. These properties of TLP, resembling those of other filamentous viruses, as well as their presence in infected tissues only, lend strength to the suggestion that TLP are the causal agent of the tristeza disease. © 1972.
Scientific Publication
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