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Preparation and epitope characterization of monoclonal antibodies suitable for detection of Tomato yellow leaf curl virus
Year:
2010
Source of publication :
Phytoparasitica
Authors :
Gafni, Yedidya
;
.
Zrachya, Avi
;
.
Volume :
38
Co-Authors:
Solmesky, L.J., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Zrachya, A., Department of Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Denisova, G., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel
Gershoni, J.M., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
201
To page:
208
(
Total pages:
8
)
Abstract:
Tomato yellow leaf curl virus (TYLCV) is a begomovirus that seriously threatens tomato crops worldwide. Current immunodiagnostic methods for this pathogen employ commercially produced mAbs raised against TYLCV. However, despite the existence of these mAbs, little information regarding their characterization or strategy of production has been published. In addition, research on TYLCV would certainly benefit were more mAbs available, thus allowing efficient examination of the virus life cycle, modes of pathogenesis and possibly the development of resistant cultivars. The coat protein (CP) of TYLCV is the only known building block of the viral capsid. Therefore, in this study we used CP as an immunogen for the production of novel mAbs. We employed a strategy in which the CP was truncated at its C-terminus to avoid intra- and inter-protein interactions that could impair epitope exposure. For the same reason, we used a denaturated antigen to expose linear epitopes during the immunization. This effort yielded three mAbs: they were characterized biochemically and immunologically, and their epitopes were mapped. Possible applications of these mAbs are discussed. © 2010 Springer Science + Business Media BV.
Note:
Related Files :
Begomovirus
Capsid protein
Epitope mapping
Immunogen
Miridae
Tomato yellow leaf curl virus
TYLCV
Show More
Related Content
More details
DOI :
10.1007/s12600-010-0089-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30490
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
Scientific Publication
Preparation and epitope characterization of monoclonal antibodies suitable for detection of Tomato yellow leaf curl virus
38
Solmesky, L.J., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Zrachya, A., Department of Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel
Denisova, G., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel
Gershoni, J.M., Department of Cell Research and Immunology, Tel-Aviv University, Tel Aviv 69978, Israel
Preparation and epitope characterization of monoclonal antibodies suitable for detection of Tomato yellow leaf curl virus
Tomato yellow leaf curl virus (TYLCV) is a begomovirus that seriously threatens tomato crops worldwide. Current immunodiagnostic methods for this pathogen employ commercially produced mAbs raised against TYLCV. However, despite the existence of these mAbs, little information regarding their characterization or strategy of production has been published. In addition, research on TYLCV would certainly benefit were more mAbs available, thus allowing efficient examination of the virus life cycle, modes of pathogenesis and possibly the development of resistant cultivars. The coat protein (CP) of TYLCV is the only known building block of the viral capsid. Therefore, in this study we used CP as an immunogen for the production of novel mAbs. We employed a strategy in which the CP was truncated at its C-terminus to avoid intra- and inter-protein interactions that could impair epitope exposure. For the same reason, we used a denaturated antigen to expose linear epitopes during the immunization. This effort yielded three mAbs: they were characterized biochemically and immunologically, and their epitopes were mapped. Possible applications of these mAbs are discussed. © 2010 Springer Science + Business Media BV.
Scientific Publication
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