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Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms
Year:
2015
Source of publication :
BMC Genomics
Authors :
Dagar, Anurag
;
.
Friedman, Haya
;
.
Lurie, Susan
;
.
Volume :
16
Co-Authors:

Puig, C.P., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Dagar, A., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Marti Ibanez, C., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Singh, V., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Crisosto, C.H., University of California Davis, Plant Sciences Department, 1 Shields Ave, Davis, CA, United States
Friedman, H., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Lurie, S., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Granell, A., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
 

Facilitators :
From page:
245
To page:
0
(
Total pages:
-244
)
Abstract:
Background: Cold storage induces chilling injury (CI) disorders in peach fruit (woolliness/mealiness, flesh browning and reddening/bleeding) manifested when ripened at shelf life. To gain insight into the mechanisms underlying CI, we analyzed the transcriptome of 'Oded' (high tolerant) and 'Hermoza' (relatively tolerant to woolliness, but sensitive to browning and bleeding) peach cultivars at pre-symptomatic stages. The expression profiles were compared and validated with two previously analyzed pools (high and low sensitive to woolliness) from the Pop-DG population. The four fruit types cover a wide range of sensitivity to CI. The four fruit types were also investigated with the ROSMETER that provides information on the specificity of the transcriptomic response to oxidative stress. Results: We identified quantitative differences in a subset of core cold responsive genes that correlated with sensitivity or tolerance to CI at harvest and during cold storage, and also subsets of genes correlating specifically with high sensitivity to woolliness and browning. Functional analysis indicated that elevated levels, at harvest and during cold storage, of genes related to antioxidant systems and the biosynthesis of metabolites with antioxidant activity correlates with tolerance. Consistent with these results, ROSMETER analysis revealed oxidative stress in 'Hermoza' and the progeny pools, but not in the cold resistant 'Oded'. By contrast, cold storage induced, in sensitivity to woolliness dependant manner, a gene expression program involving the biosynthesis of secondary cell wall and pectins. Furthermore, our results indicated that while ethylene is related to CI tolerance, differential auxin subcellular accumulation and signaling may play a role in determining chilling sensitivity/tolerance. In addition, sugar partitioning and demand during cold storage may also play a role in the tolerance/sensitive mechanism. The analysis also indicates that vesicle trafficking, membrane dynamics and cytoskeleton organization could have a role in the tolerance/sensitive mechanism. In the case of browning, our results suggest that elevated acetaldehyde related genes together with the core cold responses may increase sensitivity to browning in shelf life. Conclusions: Our data suggest that in sensitive fruit a cold response program is activated and regulated by auxin distribution and ethylene and these hormones have a role in sensitivity to CI even before fruit are cold stored. © Puig et al.
Note:
Related Files :
biosynthesis
cold tolerance
ethylene
Genetics
metabolism
Prunus persica
reactive oxygen species
shelf life
Show More
Related Content
More details
DOI :
10.1186/s12864-015-1395-6
Article number:
245
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30501
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
Scientific Publication
Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms
16

Puig, C.P., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Dagar, A., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Marti Ibanez, C., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
Singh, V., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Crisosto, C.H., University of California Davis, Plant Sciences Department, 1 Shields Ave, Davis, CA, United States
Friedman, H., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Lurie, S., Agricultural Research Organization, Volcani Center, Department of Postharvest Science of Fresh Produce, P.O. Box 6, Bet Dagan, Israel
Granell, A., CSIC-Universidad Politecnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
 

Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms
Background: Cold storage induces chilling injury (CI) disorders in peach fruit (woolliness/mealiness, flesh browning and reddening/bleeding) manifested when ripened at shelf life. To gain insight into the mechanisms underlying CI, we analyzed the transcriptome of 'Oded' (high tolerant) and 'Hermoza' (relatively tolerant to woolliness, but sensitive to browning and bleeding) peach cultivars at pre-symptomatic stages. The expression profiles were compared and validated with two previously analyzed pools (high and low sensitive to woolliness) from the Pop-DG population. The four fruit types cover a wide range of sensitivity to CI. The four fruit types were also investigated with the ROSMETER that provides information on the specificity of the transcriptomic response to oxidative stress. Results: We identified quantitative differences in a subset of core cold responsive genes that correlated with sensitivity or tolerance to CI at harvest and during cold storage, and also subsets of genes correlating specifically with high sensitivity to woolliness and browning. Functional analysis indicated that elevated levels, at harvest and during cold storage, of genes related to antioxidant systems and the biosynthesis of metabolites with antioxidant activity correlates with tolerance. Consistent with these results, ROSMETER analysis revealed oxidative stress in 'Hermoza' and the progeny pools, but not in the cold resistant 'Oded'. By contrast, cold storage induced, in sensitivity to woolliness dependant manner, a gene expression program involving the biosynthesis of secondary cell wall and pectins. Furthermore, our results indicated that while ethylene is related to CI tolerance, differential auxin subcellular accumulation and signaling may play a role in determining chilling sensitivity/tolerance. In addition, sugar partitioning and demand during cold storage may also play a role in the tolerance/sensitive mechanism. The analysis also indicates that vesicle trafficking, membrane dynamics and cytoskeleton organization could have a role in the tolerance/sensitive mechanism. In the case of browning, our results suggest that elevated acetaldehyde related genes together with the core cold responses may increase sensitivity to browning in shelf life. Conclusions: Our data suggest that in sensitive fruit a cold response program is activated and regulated by auxin distribution and ethylene and these hormones have a role in sensitivity to CI even before fruit are cold stored. © Puig et al.
Scientific Publication
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