Co-Authors:
Herbette, S., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Le Menn, A., IUT Génie Biologique, Université d'Avignon, 84029 Avignon, France, UR GAFL, INRA, 84143 Montfavet, France
Rousselle, P., UR GAFL, INRA, 84143 Montfavet, France
Ameglio, T., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Faltin, Z., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Branlard, G., UMR 1095-ASP, INRA, Université Blaise Pascal, 63039 Clermont-Ferrand, France
Eshdat, Y., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, Volcani Center, 50250 Bet-Dagan, Israel
Julien, J.-L., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Drevet, J.R., UMR 6547-GEEM, CNRS, Université Blaise Pascal, 63177 Aubière, France
Roeckel-Drevet, P., UMR 547-PIAF, INRA, Université Blaise Pascal, 63177 Aubière, France
Abstract:
To investigate the function of glutathione peroxidase (GPX) in plants, we produced transgenic tomato plants overexpressing an eukaryotic selenium-independent GPX (GPX5). We show here that total GPX activity was increased by 50% in transgenic plants, when compared to control plants transformed with the binary vector without the insert (PZP111). A preliminary two-dimensional electrophoretic protein analysis of the GPX overexpressing plants showed notably a decrease in the accumulation of proteins identified as rubisco small subunit 1 and fructose-1,6-bisphosphate aldolase, two proteins involved in photosynthesis. These observations, together with the fact that in standard culture conditions, GPX-overexpressing plants were not phenotypically distinct from control plants prompted us to challenge the plants with a chilling treatment that is known to affect photosynthesis activity. We found that upon chilling treatment with low light level, photosynthesis was not affected in GPX-overexpressing plants while it was in control plants, as revealed by chlorophyll fluorescence parameters and fructose-1,6-biphosphatase activity. These results suggest that overexpression of a selenium-independent GPX in tomato plants modifies specifically gene expression and leads to modifications of photosynthetic regulation processes. © 2005 Elsevier B.V. All rights reserved.
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