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Expressing yeast SAMdc gene confers broad changes in gene expression and alters fatty acid composition in tomato fruit
Year:
2011
Source of publication :
Physiologia Plantarum
Authors :
Bar-Or, Carmiya
;
.
Chen, Lea
;
.
Kolotilin, Igor
;
.
Koltai, Hinanit
;
.
Levin, Ilan
;
.
Nahon, Saadia
;
.
Reuveni, Moshe
;
.
Shlomo, Haviva
;
.
Volume :
142
Co-Authors:
Kolotilin, I., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Koltai, H., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Bar-Or, C., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Chen, L., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Nahon, S., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Shlomo, H., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Levin, I., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Reuveni, M., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
211
To page:
223
(
Total pages:
13
)
Abstract:
Tomato (Solanum lycopersicum) fruits expressing a yeast S-adenosyl methionine decarboxylase (ySAMdc) gene under control of a ripening-induced promoter show altered phytonutrient content and broad changes in gene expression. Genome-wide transcriptional alterations in pericarp tissues of the ySAMdc-expressing fruits are shown. Consistent with the ySAMdc expression pattern from the ripening-induced promoter, very minor transcriptional alterations were detected at the mature green developmental stage. At the breaker and red stages, altered levels of numerous transcripts were observed with a general tendency toward upregulation in the transgenic fruits. Ontological analysis of up- and downregulated transcript groups revealed various affected metabolic processes, mainly carbohydrate and amino acid metabolism, and protein synthesis, which appeared to be intensified in the ripening transgenic fruits. Other functional ontological categories of altered transcripts represented signal transduction, transcription regulation, RNA processing, molecular transport and stress response, as well as metabolism of lipids, glycans, xenobiotics, energy, cofactors and vitamins. In addition, transcript levels of genes encoding structural enzymes for several biosynthetic pathways showed strong correlations to levels of specific metabolites that displayed altered levels in transgenic fruits. Increased transcript levels of fatty acid biosynthesis enzymes were accompanied by a change in the fatty acid profile of transgenic fruits, most notably increasing ω-3 fatty acids at the expense of other lipids. Thus, SAMdc is a prime target in manipulating the nutritional value of tomato fruits. Combined with analyses of selected metabolites in the overripe fruits, a model of enhanced homeostasis of the pericarp tissue in the polyamine-accumulating tomatoes is proposed. © Physiologia Plantarum 2011.
Note:
Related Files :
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fatty acids
Genetics
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light
metabolism
Solanum
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More details
DOI :
10.1111/j.1399-3054.2011.01458.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30527
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Expressing yeast SAMdc gene confers broad changes in gene expression and alters fatty acid composition in tomato fruit
142
Kolotilin, I., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Koltai, H., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Bar-Or, C., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Chen, L., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Nahon, S., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Shlomo, H., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Levin, I., Vegetable Research Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Reuveni, M., Ornamental Horticulture Department, Institute of Plant Sciences, ARO Volcani Center, Bet Dagan 50250, Israel
Expressing yeast SAMdc gene confers broad changes in gene expression and alters fatty acid composition in tomato fruit
Tomato (Solanum lycopersicum) fruits expressing a yeast S-adenosyl methionine decarboxylase (ySAMdc) gene under control of a ripening-induced promoter show altered phytonutrient content and broad changes in gene expression. Genome-wide transcriptional alterations in pericarp tissues of the ySAMdc-expressing fruits are shown. Consistent with the ySAMdc expression pattern from the ripening-induced promoter, very minor transcriptional alterations were detected at the mature green developmental stage. At the breaker and red stages, altered levels of numerous transcripts were observed with a general tendency toward upregulation in the transgenic fruits. Ontological analysis of up- and downregulated transcript groups revealed various affected metabolic processes, mainly carbohydrate and amino acid metabolism, and protein synthesis, which appeared to be intensified in the ripening transgenic fruits. Other functional ontological categories of altered transcripts represented signal transduction, transcription regulation, RNA processing, molecular transport and stress response, as well as metabolism of lipids, glycans, xenobiotics, energy, cofactors and vitamins. In addition, transcript levels of genes encoding structural enzymes for several biosynthetic pathways showed strong correlations to levels of specific metabolites that displayed altered levels in transgenic fruits. Increased transcript levels of fatty acid biosynthesis enzymes were accompanied by a change in the fatty acid profile of transgenic fruits, most notably increasing ω-3 fatty acids at the expense of other lipids. Thus, SAMdc is a prime target in manipulating the nutritional value of tomato fruits. Combined with analyses of selected metabolites in the overripe fruits, a model of enhanced homeostasis of the pericarp tissue in the polyamine-accumulating tomatoes is proposed. © Physiologia Plantarum 2011.
Scientific Publication
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