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Analysis of microtubule-associated-proteins during IBA-mediated adventitious root induction reveals KATANIN dependent and independent alterations of expression patterns
Year:
2015
Source of publication :
PLoS ONE
Authors :
Abu-Abied, Mohamad
;
.
Mordehaev, Inna
;
.
Ophir, Ron
;
.
Sadot, Einat
;
.
Sunil Kumar, Gujulla B.
;
.
Volume :
10
Co-Authors:
Abu-Abied, M., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Mordehaev, I., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Sunil Kumar, G.B., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Ophir, R., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Wasteneys, G.O., Department of Botany, University of British Columbia, Vancouver, BC, Canada
Sadot, E., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Department of Botany, University of British Columbia, Vancouver, BC, Canada
Facilitators :
From page:
0
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Total pages:
1
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Abstract:
Adventitious roots (AR) are post embryonic lateral organs that differentiate from non-root tissues. The understanding of the molecular mechanism which underlies their differentiation is important because of their central role in vegetative plant propagation. Here it was studied how the expression of different microtubule (MT)-associated proteins (MAPs) is affected during AR induction, and whether expression differences are dependent on MT organization itself. To examine AR formation when MTs are disturbed we used two mutants in the MT severing protein KATANIN. It was found that rate and number of AR primordium formed following IBA induction for three days was reduced in bot1-1 and bot1-7 plants. The reduced capacity to form ARs in bot1-1 was associated with altered expression of MAP-encoding genes along AR induction. While the expression of MAP65-4, MAP65-3, AURORA1, AURORA2 and TANGLED, increased in wild-type but not in bot1-1 plants, the expression of MAP65-8 and MDP25 decreased in wild type plants but not in the bot1-1 plant after two days of IBA-treatment. The expression of MOR1 was increased two days after AR induction in wild type and bot1-1 plants. To examine its expression specifically in AR primordium, MOR1 upstream regulatory sequence was isolated and cloned to regulate GFP. Expression of GFP was induced in the primary root tips and lateral roots, in the pericycle of the hypocotyls and in all stages of AR primordium formation. It is concluded that the expression of MAPs is regulated along AR induction and that reduction in KATANIN expression inhibits AR formation and indirectly influences the specific expression of some MAPs. © 2015 Abu-Abied et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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More details
DOI :
10.1371/journal.pone.0143828
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30545
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Analysis of microtubule-associated-proteins during IBA-mediated adventitious root induction reveals KATANIN dependent and independent alterations of expression patterns
10
Abu-Abied, M., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Mordehaev, I., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Sunil Kumar, G.B., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Ophir, R., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Wasteneys, G.O., Department of Botany, University of British Columbia, Vancouver, BC, Canada
Sadot, E., Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Institute of Plant Sciences, Volcani Center, ARO, Bet-Dagan, Israel
Department of Botany, University of British Columbia, Vancouver, BC, Canada
Analysis of microtubule-associated-proteins during IBA-mediated adventitious root induction reveals KATANIN dependent and independent alterations of expression patterns
Adventitious roots (AR) are post embryonic lateral organs that differentiate from non-root tissues. The understanding of the molecular mechanism which underlies their differentiation is important because of their central role in vegetative plant propagation. Here it was studied how the expression of different microtubule (MT)-associated proteins (MAPs) is affected during AR induction, and whether expression differences are dependent on MT organization itself. To examine AR formation when MTs are disturbed we used two mutants in the MT severing protein KATANIN. It was found that rate and number of AR primordium formed following IBA induction for three days was reduced in bot1-1 and bot1-7 plants. The reduced capacity to form ARs in bot1-1 was associated with altered expression of MAP-encoding genes along AR induction. While the expression of MAP65-4, MAP65-3, AURORA1, AURORA2 and TANGLED, increased in wild-type but not in bot1-1 plants, the expression of MAP65-8 and MDP25 decreased in wild type plants but not in the bot1-1 plant after two days of IBA-treatment. The expression of MOR1 was increased two days after AR induction in wild type and bot1-1 plants. To examine its expression specifically in AR primordium, MOR1 upstream regulatory sequence was isolated and cloned to regulate GFP. Expression of GFP was induced in the primary root tips and lateral roots, in the pericycle of the hypocotyls and in all stages of AR primordium formation. It is concluded that the expression of MAPs is regulated along AR induction and that reduction in KATANIN expression inhibits AR formation and indirectly influences the specific expression of some MAPs. © 2015 Abu-Abied et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Scientific Publication
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