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אסיף מאגר המחקר החקלאי
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Advanced technologies for parasitic weed control
Year:
2012
Source of publication :
Weed Science
Authors :
Aly, Radi
;
.
Volume :
60
Co-Authors:
Aly, R., Department of Plant Disease and Weed Research, ARO, Newe-Yaar Research Center, P.O. Box 1021, Ramat Yeshai 30095, Israel
Facilitators :
From page:
290
To page:
294
(
Total pages:
5
)
Abstract:
Parasitic weeds such as Phelipanche and Orobanche are obligate holoparasites that attack roots of almost all economically important crops in semiarid regions of the world. A wide variety of parasitic weed control strategies (chemical, biological, cultural, and resistant crops) has been tried. Unfortunately, most are partially effective and have significant limitations. The current mini review will discuss the needs for alternative methods and will summarize current and new biotechnology-based approaches for broomrape control. At present, we have generated transgenic tobacco plants expressing a cecropin peptide (sarcotoxin IA), under the control of the inducible HMG2 promoter. Transgenic lines enhanced host resistance to the parasitic weed; transgenes showed higher numbers of aborted parasitization events, reduced Phelipanche biomass, and increased host biomass. Sarcotoxin IA had no obvious effect on growth and development of transgenic host plants. Mannitol content in the parasite is regulated by Mannose 6-Phosphate Reductase (M6PR) gene, an essential process to broomrape species for water and nutrient uptake from the host. In our study, we used the inverted repeat technique to silence the parasite target gene, M6PR. In this study it was shown that the endogenous M6PR mRNA from P. aegyptiaca tubercles or shoots grown on transgenic tomato plants harboring the M6PR silencing construct was reduced by 60 to 80%. The number of dead tubercles was also increased significantly on transgenic plants as compared with the control plants. The strategies presented here are potentially superior to other methods in that they are effective, have a low cost of implementation for producers, and are safe for the environment. © 2012 Weed Science Society of America.
Note:
Related Files :
Agricultural economics
biotechnology
gene expression
growth response
Mannose 6-phosphate reductase
Orobanche
Show More
Related Content
More details
DOI :
10.1614/WS-D-11-00066.1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30592
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Advanced technologies for parasitic weed control
60
Aly, R., Department of Plant Disease and Weed Research, ARO, Newe-Yaar Research Center, P.O. Box 1021, Ramat Yeshai 30095, Israel
Advanced technologies for parasitic weed control
Parasitic weeds such as Phelipanche and Orobanche are obligate holoparasites that attack roots of almost all economically important crops in semiarid regions of the world. A wide variety of parasitic weed control strategies (chemical, biological, cultural, and resistant crops) has been tried. Unfortunately, most are partially effective and have significant limitations. The current mini review will discuss the needs for alternative methods and will summarize current and new biotechnology-based approaches for broomrape control. At present, we have generated transgenic tobacco plants expressing a cecropin peptide (sarcotoxin IA), under the control of the inducible HMG2 promoter. Transgenic lines enhanced host resistance to the parasitic weed; transgenes showed higher numbers of aborted parasitization events, reduced Phelipanche biomass, and increased host biomass. Sarcotoxin IA had no obvious effect on growth and development of transgenic host plants. Mannitol content in the parasite is regulated by Mannose 6-Phosphate Reductase (M6PR) gene, an essential process to broomrape species for water and nutrient uptake from the host. In our study, we used the inverted repeat technique to silence the parasite target gene, M6PR. In this study it was shown that the endogenous M6PR mRNA from P. aegyptiaca tubercles or shoots grown on transgenic tomato plants harboring the M6PR silencing construct was reduced by 60 to 80%. The number of dead tubercles was also increased significantly on transgenic plants as compared with the control plants. The strategies presented here are potentially superior to other methods in that they are effective, have a low cost of implementation for producers, and are safe for the environment. © 2012 Weed Science Society of America.
Scientific Publication
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