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Effects of various elicitors on the transcription of a β-1, 3-endoglucanase gene in citrus fruit
Year:
2002
Source of publication :
Journal of Phytopathology
Authors :
Droby, Samir
;
.
Porat, Ron
;
.
Vinokur, Victor
;
.
Volume :
150
Co-Authors:

Porat, R., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
McCollum, T.G., USDA, USHRL 2001 S. Rock Road, Ft. Pierce, FL 34945, USA
Vinokur, V., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Droby, S., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel

Facilitators :
From page:
70
To page:
75
(
Total pages:
6
)
Abstract:
Very little is yet known regarding the molecular mechanisms involved in pathogen defense responses in citrus fruit. Recently, a basic β-1, 3-endoglucanase (EC 3.2.2.39) belonging to the pathogenesis-related (PR) group of proteins, has been purified from Citrus sinensis (L) Osbeck cv. 'Valencia' orange callus. Specific antibodies raised against the purified protein were used to screen 'Valencia' callus and flavedo cDNA expression libraries, and to isolate its corresponding cDNA, designated gns1. The gns1 gene encodes a predicted polypeptide of 336 amino acids with a molecular mass of 37.3 kDa and a basic pI of 9.19, and shares 55-65% identity with several other plant β-1, 3-endoglucanase proteins. Hereby, we show that the expression of the gns1 gene is markedly induced by wounding and inoculation with Penicillium digitatum (Pers. Fr.) Sacc., and following treatments with various elicitors that induce fruit resistance against P. digitatum. These treatments include UV irradiation, application of jasmonic acid (JA), β-aminobutyric acid (BABA), Candida oleophila antagonist yeast cells and hot water rinsing and brushing. Overall, based on various RNA gel blot hybridizations, we assume that gns1 is most likely to be part of the molecular mechanisms involved in pathogen defense responses in citrus fruit.
Note:
Related Files :
candida
Candida oleophila
Citrus sinensis
disease resistance
Embryophyta
Eukaryota
Genetics
Pathogen resistance
Penicillium
Show More
Related Content
More details
DOI :
10.1046/j.1439-0434.2002.00719.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30595
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
You may also be interested in
Scientific Publication
Effects of various elicitors on the transcription of a β-1, 3-endoglucanase gene in citrus fruit
150

Porat, R., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
McCollum, T.G., USDA, USHRL 2001 S. Rock Road, Ft. Pierce, FL 34945, USA
Vinokur, V., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Droby, S., Dept. Postharvest Sci. Fresh Produce, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel

Effects of various elicitors on the transcription of a β-1, 3-endoglucanase gene in citrus fruit
Very little is yet known regarding the molecular mechanisms involved in pathogen defense responses in citrus fruit. Recently, a basic β-1, 3-endoglucanase (EC 3.2.2.39) belonging to the pathogenesis-related (PR) group of proteins, has been purified from Citrus sinensis (L) Osbeck cv. 'Valencia' orange callus. Specific antibodies raised against the purified protein were used to screen 'Valencia' callus and flavedo cDNA expression libraries, and to isolate its corresponding cDNA, designated gns1. The gns1 gene encodes a predicted polypeptide of 336 amino acids with a molecular mass of 37.3 kDa and a basic pI of 9.19, and shares 55-65% identity with several other plant β-1, 3-endoglucanase proteins. Hereby, we show that the expression of the gns1 gene is markedly induced by wounding and inoculation with Penicillium digitatum (Pers. Fr.) Sacc., and following treatments with various elicitors that induce fruit resistance against P. digitatum. These treatments include UV irradiation, application of jasmonic acid (JA), β-aminobutyric acid (BABA), Candida oleophila antagonist yeast cells and hot water rinsing and brushing. Overall, based on various RNA gel blot hybridizations, we assume that gns1 is most likely to be part of the molecular mechanisms involved in pathogen defense responses in citrus fruit.
Scientific Publication
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