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Development of a 25-plex SNP assay for traceability in cattle
Year:
2009
Source of publication :
Animal Genetics
Authors :
Karniol, Baruch
;
.
Ron, Micha
;
.
Seroussi, Eyal
;
.
Shirak, Andrey
;
.
Weller, Joel Ira
;
.
Volume :
40
Co-Authors:
Karniol, B., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Shirak, A., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Baruch, E., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Singrün, C., Agrobiogen GmbH, Hilgertshausen-Tandern 86567, Germany
Tal, A., Bactochem Ltd, Ness-Ziona 74031, Israel
Cahana, A., Bactochem Ltd, Ness-Ziona 74031, Israel
Kam, M., Bactochem Ltd, Ness-Ziona 74031, Israel
Skalski, Y., Bactochem Ltd, Ness-Ziona 74031, Israel
Brem, G., Agrobiogen GmbH, Hilgertshausen-Tandern 86567, Germany
Weller, J.I., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Ron, M., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Seroussi, E., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel, Institute of Animal Sciences, ARO, Volcani Center, Bet-Dagan 50250, Israel
Facilitators :
From page:
353
To page:
356
(
Total pages:
4
)
Abstract:
Summary Single nucleotide polymorphisms (SNPs) are amenable to automation and therefore have become the marker of choice for DNA profiling. SNaPshot, a primer extension-based method, was used to multiplex 25 SNPs that have been previously validated as useful for identity control. Detection of extended products was based on four different fluorochromes and extension primers with oligonucleotide tails of differing lengths, thus controlling the concise length of the entire chromatogram to 81 bases. Allele frequencies for Holstein, Simmental, Limousin, Angus, Charolais and Tux Cattle were estimated and significant positive Pearson-correlation coefficients were obtained among the analysed breeds. The probability that two randomly unrelated individuals would share identical genotypes for all 25 loci varied from 10-8 to 10 -10 for these breeds. For parentage control, the exclusion power was found to be 99.9% when the genotypes of both putative parents are known. A traceability test of duplicated samples indicated a high genotyping precision of >0.998. This was further corroborated by analysis of 60 cases of parent-sib pairs and trio families. The 25-plex SNaPshot assay is adapted for low- and high-throughput capacity and thus presents an alternative for DNA-based traceability in the major commercial cattle breeds. © 2009 International Society for Animal Genetics.
Note:
Related Files :
Animal
animal disease
Animals
cattle
chemistry
DNA
Female
gene frequency
Genetics
Multiplex
Polymerase Chain Reaction
Show More
Related Content
More details
DOI :
10.1111/j.1365-2052.2008.01846.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30608
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
Scientific Publication
Development of a 25-plex SNP assay for traceability in cattle
40
Karniol, B., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Shirak, A., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Baruch, E., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Singrün, C., Agrobiogen GmbH, Hilgertshausen-Tandern 86567, Germany
Tal, A., Bactochem Ltd, Ness-Ziona 74031, Israel
Cahana, A., Bactochem Ltd, Ness-Ziona 74031, Israel
Kam, M., Bactochem Ltd, Ness-Ziona 74031, Israel
Skalski, Y., Bactochem Ltd, Ness-Ziona 74031, Israel
Brem, G., Agrobiogen GmbH, Hilgertshausen-Tandern 86567, Germany
Weller, J.I., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Ron, M., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel
Seroussi, E., Agricultural Research Organization, Institute of Animal Science, Bet-Dagan 50250, Israel, Institute of Animal Sciences, ARO, Volcani Center, Bet-Dagan 50250, Israel
Development of a 25-plex SNP assay for traceability in cattle
Summary Single nucleotide polymorphisms (SNPs) are amenable to automation and therefore have become the marker of choice for DNA profiling. SNaPshot, a primer extension-based method, was used to multiplex 25 SNPs that have been previously validated as useful for identity control. Detection of extended products was based on four different fluorochromes and extension primers with oligonucleotide tails of differing lengths, thus controlling the concise length of the entire chromatogram to 81 bases. Allele frequencies for Holstein, Simmental, Limousin, Angus, Charolais and Tux Cattle were estimated and significant positive Pearson-correlation coefficients were obtained among the analysed breeds. The probability that two randomly unrelated individuals would share identical genotypes for all 25 loci varied from 10-8 to 10 -10 for these breeds. For parentage control, the exclusion power was found to be 99.9% when the genotypes of both putative parents are known. A traceability test of duplicated samples indicated a high genotyping precision of >0.998. This was further corroborated by analysis of 60 cases of parent-sib pairs and trio families. The 25-plex SNaPshot assay is adapted for low- and high-throughput capacity and thus presents an alternative for DNA-based traceability in the major commercial cattle breeds. © 2009 International Society for Animal Genetics.
Scientific Publication
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