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Assessment of genetic diversity of Xanthomonas campestris pv. campestris isolates from Israel by various DNA fingerprinting techniques
Year:
2007
Source of publication :
Plant Pathology
Authors :
Dagar, Anurag
;
.
Volume :
56
Co-Authors:
Valverde, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Department of Vegetal Production, IRNASA-CSIC, Salamanca 37008, Spain
Hubert, T., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Stolov, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Dagar, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Kopelowitz, J., Savyon Diagnostics Ltd., 3 Habosem St, Ashdod 77610, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Facilitators :
From page:
17
To page:
25
(
Total pages:
9
)
Abstract:
Three molecular typing methods were used to investigate genetic diversity among Xanthomonas campestris pv. campestris isolates obtained in Israel and others previously obtained from different geographical locations (collection isolates). Using pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and repetitive sequence-based PCR (rep-PCR), 22 different isolates were divided into 11, 12 and 13 differentiated genotypes, respectively. All collection isolates yielded different genotypes and, among the isolates from Israel, several new genotypes were found. These findings not only support the observed heterogeneity within X. campestris pv. campestris, but also suggest that variability at the genomic level in this pathovar is higher than previously estimated. Moreover, while previous studies suggested that PCR patterns obtained with integron-specific primers are conserved in most X. campestris pathovars, PCR patterns of this element yielded four different types among the X. campestris pv. campestris isolates tested, thus supporting the relatively high diversity in this pathovar. Although differences in pathogenicity were observed among isolates, assays using cauliflower and radish did not indicate a correlation between pathogenicity and genotype. © 2006 The Authors.
Note:
Related Files :
bacterium
isolated population
Israel
Polymerase Chain Reaction
Raphanus sativus
Xanthomonas campestris pv. campestris
Show More
Related Content
More details
DOI :
10.1111/j.1365-3059.2006.01456.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30627
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
Assessment of genetic diversity of Xanthomonas campestris pv. campestris isolates from Israel by various DNA fingerprinting techniques
56
Valverde, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel, Department of Vegetal Production, IRNASA-CSIC, Salamanca 37008, Spain
Hubert, T., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Stolov, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Dagar, A., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Kopelowitz, J., Savyon Diagnostics Ltd., 3 Habosem St, Ashdod 77610, Israel
Burdman, S., Department of Plant Pathology and Microbiology, Faculty of Agricultural, Food and Environmental Quality Sciences, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
Assessment of genetic diversity of Xanthomonas campestris pv. campestris isolates from Israel by various DNA fingerprinting techniques
Three molecular typing methods were used to investigate genetic diversity among Xanthomonas campestris pv. campestris isolates obtained in Israel and others previously obtained from different geographical locations (collection isolates). Using pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) and repetitive sequence-based PCR (rep-PCR), 22 different isolates were divided into 11, 12 and 13 differentiated genotypes, respectively. All collection isolates yielded different genotypes and, among the isolates from Israel, several new genotypes were found. These findings not only support the observed heterogeneity within X. campestris pv. campestris, but also suggest that variability at the genomic level in this pathovar is higher than previously estimated. Moreover, while previous studies suggested that PCR patterns obtained with integron-specific primers are conserved in most X. campestris pathovars, PCR patterns of this element yielded four different types among the X. campestris pv. campestris isolates tested, thus supporting the relatively high diversity in this pathovar. Although differences in pathogenicity were observed among isolates, assays using cauliflower and radish did not indicate a correlation between pathogenicity and genotype. © 2006 The Authors.
Scientific Publication
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