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Elucidation of pectinolytic enzyme activities of a non-pathogenic watermelon pathogen mutant, Fusarium oxysporum f.sp. niveum M87
Year:
2007
Source of publication :
Australasian Plant Pathology
Authors :
Denisov, Youlia
;
.
Freeman, Stanley
;
.
Volume :
36
Co-Authors:
Basaran, P., Department of Food Engineering, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey, Institute for Prospective Technological Studies (IPTS), European Commission-Joint Research Center, Edificio Expo, Sevilla, Spain
Ozcan, M., Department of Food Engineering, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey
Denisov, Y., Department of Plant Pathology, Agricultural Research Organization, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, Agricultural Research Organization, ARO, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
135
To page:
141
(
Total pages:
7
)
Abstract:
The watermelon pathogen Fusarium oxysporum f.sp. niveum Fon2 and its UV-induced non-virulent mutant M87 were evaluated for the extracellular production of polygalacturolitic enzymes. The mutant M87 was found to lack two functional exopolygalacturonases (exoPG). The exoPG proteins of the Fon2 strain had similar molecular masses of 50 kDa and 49 kDa, and showed significant homology with exoPGs of the tomato pathogen, F. oxysporum f.sp. lycopersici. Two isoforms of an endoPG were also detected in both Fon2 and M87 using SDS-PAGE. Using reverse transcriptase?polymerase chain reaction, specific oligonucleotides designed from the coding region of the exoPG genes determined to be present in Fon2 were used to investigate the presence of transcripts in the cultures grown in induction media. The presence of mRNAs of both exoPGs were detected when Fon2 and the mutant M87 were grown with pectin, sodium polygalacturonate and polygalacturonic acid as sole carbon sources, but not glucose. Expression of both exoPGs in infected watermelon seedlings was observed until plants fully developed infection symptoms with Fon2 on day 6; M87-infected plants showed no symptoms. © Australasian Plant Pathology Society 2007.
Note:
Related Files :
Citrullus lanatus var. lanatus
Fusarium oxysporum
Lycopersicon esculentum
Show More
Related Content
More details
DOI :
10.1071/AP06089
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
30635
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:56
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Scientific Publication
Elucidation of pectinolytic enzyme activities of a non-pathogenic watermelon pathogen mutant, Fusarium oxysporum f.sp. niveum M87
36
Basaran, P., Department of Food Engineering, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey, Institute for Prospective Technological Studies (IPTS), European Commission-Joint Research Center, Edificio Expo, Sevilla, Spain
Ozcan, M., Department of Food Engineering, Faculty of Agriculture, Suleyman Demirel University, Isparta, Turkey
Denisov, Y., Department of Plant Pathology, Agricultural Research Organization, ARO, Volcani Center, Bet Dagan 50250, Israel
Freeman, S., Department of Plant Pathology, Agricultural Research Organization, ARO, Volcani Center, Bet Dagan 50250, Israel
Elucidation of pectinolytic enzyme activities of a non-pathogenic watermelon pathogen mutant, Fusarium oxysporum f.sp. niveum M87
The watermelon pathogen Fusarium oxysporum f.sp. niveum Fon2 and its UV-induced non-virulent mutant M87 were evaluated for the extracellular production of polygalacturolitic enzymes. The mutant M87 was found to lack two functional exopolygalacturonases (exoPG). The exoPG proteins of the Fon2 strain had similar molecular masses of 50 kDa and 49 kDa, and showed significant homology with exoPGs of the tomato pathogen, F. oxysporum f.sp. lycopersici. Two isoforms of an endoPG were also detected in both Fon2 and M87 using SDS-PAGE. Using reverse transcriptase?polymerase chain reaction, specific oligonucleotides designed from the coding region of the exoPG genes determined to be present in Fon2 were used to investigate the presence of transcripts in the cultures grown in induction media. The presence of mRNAs of both exoPGs were detected when Fon2 and the mutant M87 were grown with pectin, sodium polygalacturonate and polygalacturonic acid as sole carbon sources, but not glucose. Expression of both exoPGs in infected watermelon seedlings was observed until plants fully developed infection symptoms with Fon2 on day 6; M87-infected plants showed no symptoms. © Australasian Plant Pathology Society 2007.
Scientific Publication
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